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ShRNA cassette sequencing from disomic and trisomic fibroblasts cultured in the presence of shRNA kinome library for 14 days

ABSTRACT: Kinome-wide shRNA screen to identify kinases differentially required for survival of cells disomic and trisomic for chromosome 21 Cells were transduced in triplicate and propagated for 14 days to allow for enrichement or depletion of shRNAs from the population

ORGANISM(S): Homo sapiens

SUBMITTER: Ahwan Pandey

PROVIDER: E-GEOD-79840 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Trisomy 21 consistently activates the interferon response.

Sullivan Kelly D KD Lewis Hannah C HC Hill Amanda A AA Pandey Ahwan A Jackson Leisa P LP Cabral Joseph M JM Smith Keith P KP Liggett L Alexander LA Gomez Eliana B EB Galbraith Matthew D MD DeGregori James J Espinosa Joaquín M JM

eLife 20160729

Although it is clear that trisomy 21 causes Down syndrome, the molecular events acting downstream of the trisomy remain ill defined. Using complementary genomics analyses, we identified the interferon pathway as the major signaling cascade consistently activated by trisomy 21 in human cells. Transcriptome analysis revealed that trisomy 21 activates the interferon transcriptional response in fibroblast and lymphoblastoid cell lines, as well as circulating monocytes and T cells. Trisomy 21 cells s ...[more]

PMID: 27472900

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