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Kinome analysis of CD151 overexpressing Jurkat T cells

ABSTRACT: The effect of CD151 expression onto the kinome of Jurkat T cells was assessed using kinome analysis. CD151 was expressed in Jurkat T cells by retroviral transduction based on a pMSCV vector. Entrez Gene: 977 UniProtKB: P48509 Jurkat T cells were transduced with the MSCV-CD151 vector and successfully transduced cells were selected using puromycin. For the kinome array experiments 3 independent samples of Jurkat cells and three independent samples of J-CD151 cells were collected. To minimize unspecific background signals, lysates from Jurkat and J-CD151 T cells harvested at different growth stages, which were then pooled to provide one sample prior to loading on the Kinexus antibody microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Olaf Kutsch

PROVIDER: E-MTAB-5978 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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