Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Tarjei Mikkelsen
PROVIDER: E-GEOD-8024 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Mikkelsen Tarjei S TS Ku Manching M Jaffe David B DB Issac Biju B Lieberman Erez E Giannoukos Georgia G Alvarez Pablo P Brockman William W Kim Tae-Kyung TK Koche Richard P RP Lee William W Mendenhall Eric E O'Donovan Aisling A Presser Aviva A Russ Carsten C Xie Xiaohui X Meissner Alexander A Wernig Marius M Jaenisch Rudolf R Nusbaum Chad C Lander Eric S ES Bernstein Bradley E BE
Nature 20070701 7153
We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find that lysine 4 and lysine 27 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably ...[more]