Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human HEK293 cells overexpressing HNF4a2 wild type or mutant forms C106R or R154X

ABSTRACT: Identification of genes regulated by the transcription factor HNF4a2 Experiment Overall Design: We used microarrays to identify genes regulated by the transcription factor HNF4a2. HEK293 cell lines containing doxycyclin-inducible wilt type or mutant forms of HNF4a2m which were introduced by FRT/FLP mediated recombination into FLP-In T-REx 293 cells (Invitrogen) were treated for 24 hours with 1 Âµg/ml doxycyclin. Gene expression profiles of induced and noninduced cells were compared to identify HNF4 regulated targets.

ORGANISM(S): Homo sapiens

SUBMITTER: Ludger Klein-Hitpass

PROVIDER: E-GEOD-8289 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

HEK293 cells overexpressing wild type HNF4a2 or mutant forms HNF4a2 C106R or HNF4a2 R154X

Project description:Analysis of gene expression in human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X, which were introduced by FRT/FLP recombination. The analysis was performed 24 hours following 1µg/ml tetracycline treatment to induce the expression of the genes. Results identify HNF4a regulated genes in kidney cells being several of these genes deregulated in renal cell carcinoma. Keywords = HEK293 embryonic kidney cells Keywords = HNF4a2 Keywords = tetracycline Keywords = FRT Keywords: ordered

2005-07-21 | GSE2700 | GEO

BioID analysis of human protein GNL3

Project description:We performed a BioID experiment in a HEK293 Flp-In T-Rex cell line that contains human GNL3 cDNA integrated at the FRT site tagged with BirA and FLAG under the control of a promoter regulated with doxycycline.

2023-12-05 | PXD045389 | Pride

Transcription profiling of human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X to identify HNF4a regulated genes in kidney cells being several of these genes deregulated in renal cell carcinoma

Project description:Analysis of gene expression in human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X, which were introduced by FRT/FLP recombination. The analysis was performed 24 hours following 1sg/ml tetracycline treatment to induce the expression of the genes. Results identify HNF4a regulated genes in kidney cells being several of these genes deregulated in renal cell carcinoma.<br><br>Note that files GSM52180.txt and GSM52184.txt as downloaded from GEO are identical.

2007-10-30 | E-GEOD-2700 | biostudies-arrayexpress

Phosphoproteome of PINK1 in HEK293 cells

Project description:triple SILAC phosphoproteomics experiment of Flp-In T-Rex HEK293 cells stably expressing either FLAG empty, PINK1-FLAG Kinase dead or PINK1-FLAG wild type were grown in ‘light’ (K0R0), ‘medium’ (K4R6) and ‘heavy’ (K8,R10) SILAC media, respectively.

2015-09-16 | PXD002127 | Pride

Co-regulation of splicing by Rbfox1 and hnRNP M [hnRNPM k-d+Rbfox1 RNA-Seq]

Project description:hnRNP M and Rbfox proteins are subunits of the Large Assembly of Splicing Regulators (LASR). The purpose of this study is to investigate how these two splicing factors affect each others' role in regulating splice site choices in pre-mRNA. hnRNP M is knocked down by RNAi in Flp-In T-REx 293 cells (Invitrogen), whereas Rbfox1 is expressed inducibly under tetracycline control from construct integrated into the genome at the FRT site. Using this system, splicing and expression profiles of cells expressing and/or lacking these proteins are compared on a whole genome level by RNA-seq technology.

2016-04-21 | GSE76476 | GEO

PP1-NIPP1 wild type and mutant overexpression in Flip-In™ T-Rex Hela cells

Project description:RNAi-seq of stable Flp-In™ T-Rex Hela cells that inducibly expressed PP1-NIPP1 and mutants.

2018-06-28 | GSE104320 | GEO

Effects of pan-UPF1 knockdown on RNA abundance

Project description:Flp-In T-REx-293 cells transfected with non-targeting control siRNA or UPF1-specific siRNA as indicated and used for total RNA-seq.

2022-04-07 | GSE176197 | GEO

Myc-dependent transcriptome of Drosophila L3 larval wing imaginal discs, and its dependency on Max and/or the PAF1 complex component Atu/Leo1

Project description:A 2-hour heat-shock at 37C was used to activate hs-FLP and an actin5C-FRT-stop-FRT-GAL4 transgene in larvae carrying any possible combination of the genetic elements UAS-Myc, UAS-Atu-IR, Max-/-. 48 hours later 16-27 wing imaginal discs were isolated from wandering L3 larvae and polyA-RNA was processed for sequencing.

2017-10-02 | E-MTAB-5470 | biostudies-arrayexpress

Uridilation by TUT4/7 restricts retrotransposition of human Line-1s

Project description:Purpose: the goal of this study was to test whether the amounts of genome-encoded Line-1s are influenced by TUTases and Mov10 Methods: RNA-Seq data were obtained for PA-1 or Hek293 Flp-IN T-Rex cells in which wild-type or mutant TUTases or Mov10 were overexpressed or the proteins were depleted by RNA interference Results: Minor changes (less than 0.4-fold) were observed in the amounts of mRNAs of Homo sapiens-specific Line-1 families in Hek293 Flp-IN T-Rex and PA-1 either overexpressing or depleted of TUTases and Mov10

2018-08-15 | GSE105264 | GEO

Characterisation of Grem1 wild-type and mutant murine pancreatic tumours

Project description:We performed RNA-seq analysis on Grem1 wild-type and Grem1-deleted pancreatic tumour tissues from Pdx1-Flp;KrasFSF-G12D;Trp53frt/frt (KPF) mice. We compared the transcriptome between these two groups of tumours with the aim to understand their molecular signatures and the relevance to human pancreatic ductal adenocarcinoma subtypes.

2022-04-09 | GSE182143 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data