Dataset Information

BioID analysis of human protein GNL3

ABSTRACT: We performed a BioID experiment in a HEK293 Flp-In T-Rex cell line that contains human GNL3 cDNA integrated at the FRT site tagged with BirA and FLAG under the control of a promoter regulated with doxycycline.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell, Kidney

SUBMITTER: Functional Proteomics Platform FPP

LAB HEAD: Cyril Ribeyre

PROVIDER: PXD045389 | Pride | 2023-12-05

REPOSITORIES: Pride

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Publications

The nucleolar protein GNL3 prevents resection of stalled replication forks.

Lebdy Rana R Canut Marine M Patouillard Julie J Cadoret Jean-Charles JC Letessier Anne A Ammar Josiane J Basbous Jihane J Urbach Serge S Miotto Benoit B Constantinou Angelos A Abou Merhi Raghida R Ribeyre Cyril C

EMBO reports 20231115 12

Faithful DNA replication requires specific proteins that protect replication forks and so prevent the formation of DNA lesions that may damage the genome. Identification of new proteins involved in this process is essential to understand how DNA lesions accumulate in cancer cells and how they tolerate them. Here, we show that human GNL3/nucleostemin, a GTP-binding protein localized mostly in the nucleolus and highly expressed in cancer cells, prevents nuclease-dependent resection of nascent DNA ...[more]

PMID: 37965896

Similar Datasets

Project description:BioID Controls for Flp-In T-REx HEK293 cells are the following; Empty Vector: VL_20151116_COT_01_HEK293_pcDNA5EV_HB VL_20151116_COT_02_HEK293_pcDNA5EV_HB VL_20151116_COT_03_HEK293_pcDNA5EV_HB VL_20151116_COT_04_HEK293_pcDNA5EV_HB BirA-Flag-EGFP: VL_20150825_COT_05_HB VL_20150825_COT_06_HB VL_20150825_COT_07_HB VL_20150825_COT_08_HB BirA-Flag-EGFP-CAAX: VL_20181019_COT_01_HB VL_20181019_COT_02_HB VL_20181019_COT_03_HB VL_20181019_COT_04_HB BioID samples for Flp-In T-REx HEK293 cells are the following; RAC1_WT: 2186_ZL_RAC1_WT_BR1_Velos1_P103_HB 2308_ZL_RAC1_WT_BR2_Velos1_HB RHOG_WT: VL_20170123_COT_01_HB VL_20170123_COT_02_HB RHOA_WT: VL_20160323_COT_04new2_HB VL_20180104_COT_01_HB CDC42_WT: VL_20180104_COT_03_HB VL_20180104_COT_04_HB RAC1_G15A: VL_20180105_COT_01_2_HB VL_20180105_COT_02_HB CDC42_G15A: VL_20180105_COT_03_HB VL_20180105_COT_04_HB RHOG_G15A: VL_20180108_COT_01_HB VL_20180108_COT_02_HB RHOA_G17A: VL_20180108_COT_03_HB VL_20180108_COT_04_HB CDC42_G12V: 2189_ZL_CDC42_G12V_BR1_Velos1_P103_HB 2263_ZL_CDC42_G12V_BR2_Velos1_HB RAC1_G12V: 1871_RAC1_G12V_BirA_Flag_HEK293_2-2_HB VL_20150825_COT_02_HB RAC2_G12V: 2199_ZL_RAC2_G12V_BR1_Velos1_P103_HB 2323_ZL_RAC2_G12V_BR2_Velos1_HB RAC3_G12V: 2200_ZL_RAC3_G12V_BR1_Velos1_P103_HB 2321_ZL_RAC3_G12V_BR2_Velos1_HB RHOA_G14V: 2190_ZL_RHOA_G14V_BR1_Velos1_P103_HB 2262_ZL_RHOA_G14V_BR2_Velos1_HB RHOB_G14V: 2191_ZL_RHOB_G14V_BR1_Velos1_P103_HB 2261_ZL_RHOB_G14V_BR2_Velos1_HB RHOBTB1_WT: 2201_ZL_RHOBTB1_WT_BR1_Velos1_P103_HB 2320_ZL_RHOBTB1_WT_BR2_Velos1_HB RHOBTB2_WT: 2202_ZL_RHOBTB2_WT_BR1_Velos1_P103_HB 2318_ZL_RHOBTB2_WT_BR2_Velos1_HB RHOC_G14V: 2192_ZL_RHOC_G14V_BR1_Velos1_P103_HB 2260_ZL_RHOC_G14V_BR2_Velos1_HB RHOD_G26V: 2193_ZL_RHOD_G26V_BR1_Velos1_P103_HB 2259_ZL_RHOD_G26V_BR2_Velos1_HB RHOF_G28V: 2246_ZL_RHOF_G28V_BR1_2194ReRun_Velos1_P103_HB 2258_ZL_RHOF_G28V_BR2_Velos1_HB RHOG_G12V: 2306_ZL_RHOG_G12V_BR2_Velos1_HB VL_20170123_COT_03_HB RHOH_WT: 2203_ZL_RHOH_WT_BR1_Velos1_P103_HB 2317_ZL_RHOH_WT_BR2_Velos1_HB RHOJ_G40V: 2195_ZL_RHOJ_G40V_BR1_Velos1_P103_HB 2257_ZL_RHOJ_G40V_BR2_Velos1_HB RHOQ_G18V: 2196_ZL_RHOQ_G18V_BR1_Velos1_P103_HB 2338_ZL_RHOQ_G18V_BR2_Velos1_HB RHOU_G58V: 2250_ZL_RHOU_G58v_BR1_2197ReRun_Velos1_P103_HB 2337_ZL_RHOU_G58V_BR2_Velos1_HB RHOV_G40V: 2198_ZL_RHOV_G40V_BR1_Velos1_P103_HB 2324_ZL_RHOV_G40V_BR2_Velos1_HB RND1_WT: 2249_ZL_RND1_WT_BR1_Velos1_P103_HB 2313_ZL_RND1_WT_BR2_Velos1_HB RND2_WT: 2247_ZL_RND2_WT_BR1_Velos1_P103_HB 2315_ZL_RND2_WT_BR2_Velos1_HB RND3_WT: VL_20151116_COT_05_HB VL_20151116_COT_06_HB BioID Controls for Flp-In T-REx HeLa cells are the following; Empty Vector: QE_20180917_COT_01_HB QE_20180917_COT_02_HB QE_20180917_COT_03_HB QE_20180917_COT_04_HB BirA-Flag-EGFP: QE_20151030_COT_02_HB QE_20151030_COT_03_HB QE_20151030_COT_04_HB QE_20151030_COT_05_HB BirA-Flag-EGFP-CAAX: QE_20181022_COT_01_HB QE_20181022_COT_02_HB QE_20181022_COT_03_HB QE_20181022_COT_04_HB BioID samples for Flp-In T-REx HeLa cells are the following; CDC42_G15A: QE_20171218_COT_05_HB QE_20171218_COT_06_HB CDC42_WT: QE_20171218_COT_01_HB QE_20171218_COT_02_HB RAC1_G15A: QE_20171218_COT_03_HB QE_20171218_COT_04_HB RAC1_WT: QE_20151102_COT_01_HB QE_20151102_COT_02_HB RHOA_G17A: QE_20171218_COT_07_HB QE_20171218_COT_08_HB RHOA_WT: QE_20171201_COT_07_HB QE_20171201_COT_08_HB RHOG_G15A: QE_20171220_COT_01_HB QE_20171220_COT_02_HB RHOG_WT: QE_20161215_COT_01_HB QE_20161215_COT_02_HB CDC42_G12V: QE_20171201_COT_09_HB QE_20171201_COT_10_HB RAC1_G12V: QE_20151030_COT_06_HB QE_20151030_COT_07_HB RAC2_G12V: QE_20180105_COT_05_HB QE_20180105_COT_06_HB RAC3_G12V: QE_20180105_COT_07_HB QE_20180105_COT_08_HB RHOA_G14V: QE_20171205_COT_17_HB QE_20171205_COT_18_HB RHOB_G14V: QE_20180105_COT_01_HB QE_20180105_COT_02_HB RHOBTB1_WT: QE_20180108_COT_07_HB QE_20180108_COT_08_HB RHOBTB2_WT: QE_20171205_COT_19_HB QE_20171205_COT_20_HB RHOC_G14V: QE_20180105_COT_03_HB QE_20180105_COT_04_HB RHOD_G26V: QE_20180108_COT_05_HB QE_20180108_COT_06_HB RHOF_G28V: QE_20171130_COT_05_HB QE_20171130_COT_06_HB RHOG_G12V: QE_20161215_COT_03_HB QE_20161215_COT_04_HB RHOH_WT: QE_20171204_COT_15_HB QE_20171204_COT_16_HB RHOJ_G40V: QE_20171130_COT_03_HB QE_20171130_COT_04_HB RHOQ_G18V: QE_20180105_COT_09_HB QE_20180105_COT_10_HB RHOU_G58V: QE_20171204_COT_11_HB QE_20171204_COT_12_new_HB RHOV_G40V: QE_20171130_COT_01_HB QE_20171130_COT_02_HB RND1_WT: QE_20180108_COT_01_HB QE_20180108_COT_02_HB RND2_WT: QE_20171204_COT_13_HB QE_20171204_COT_14_HB RND3_WT: QE_20180108_COT_03_HB QE_20180108_COT_04_HB

			Action	DRS
	GNL3_190523_HF2_B.raw	Raw
	GNL3_190523_HF2_BD.raw	Raw
	GNL3_190523_HF2_D.raw	Raw
	GNL3_190626_HF2_B.raw	Raw
	GNL3_190626_HF2_BD.raw	Raw