Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Molecular profiling of myeloid leukemia cell lines

ABSTRACT: Cell lines. The myeloid cell lines CMK, HEL, HL-60, K-562, KASUMI-1, KG-1, LAMA-84, M-07e, MONO-MAC-1, MV4-11, NB-4, OCI-AML2, OCI-AML5, SIG-M5, THP-1, and UT-7 were obtained from the DSMZ in Braunschweig, Germany (http://www.dsmz.de). The cell line ME-1 was kindly provided by Dr. Ikuya Sakai, Ehime University, Japan. Cell lines were cultured under standard conditions in RPMI 1640 containing 10% FCS, 2 mM L-glutamine, 100 units/ml penicillin and 100 units/ml streptomycin. For subsequent DNA and RNA isolation 1x107 cells of the respective cell lines were washed twice in phosphate-buffered saline, and stored as cell pellets at 800C. Gene expression profiling. Gene expression profiling (GEP) was performed essentially as described using the previously described cDNA microarray platform (Bullinger et al., N Engl J Med 2004;350:1605-1616). A reference experiement design type is where all samples are compared to a common reference. Keywords: reference_design Using regression correlation

ORGANISM(S): Homo sapiens

SUBMITTER: Lars Bullinger

PROVIDER: E-GEOD-8779 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular profiling reveals myeloid leukemia cell lines to be faithful model systems characterized by distinct genomic aberrations.

Rücker F G FG Sander S S Döhner K K Döhner H H Pollack J R JR Bullinger L L

Leukemia 20060601 6

To model and investigate different facets of leukemia pathogenesis, a widely accepted approach is to use immortalized leukemia cell lines. Although these provide powerful tools to our knowledge, few studies have addressed the question whether hematopoietic cell lines represent accurate and reliable model systems. To improve the molecular characterization of these model systems, we analyzed 17 myeloid leukemia cell lines using DNA microarray technology. By array-based comparative genomic hybridiz ...[more]

PMID: 16721385

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Dataset Information

Molecular profiling of myeloid leukemia cell lines

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Molecular profiling reveals myeloid leukemia cell lines to be faithful model systems characterized by distinct genomic aberrations.

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