Unknown,Transcriptomics,Genomics,Proteomics

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Effect of genomic imbalances on gene expression in rhabdomyosarcoma


ABSTRACT: Rhabdomyosarcoma (RMS) is the most common paediatric soft-tissue sarcoma and resembles developing skeletal muscle. Specific genomic alterations including translocations, deletions and amplification events have been associated with the alveolar and embryonal subtypes of RMS. Characterizing these changes has led to increased understanding of the underlying molecular biology. However, further aberrations and their significance remain to be defined. Genomic copy number variations have been shown to affect the level of transcription in several tumor types. In this study, we have combined array comparative genomic hybridization analysis of 13 RMS cell lines, supported by confirmatory PCR analyses, with their corresponding expression profiles. This identified sets of genes that are altered in their level of expression by genomic imbalances. We also show that the effect on gene expression was proportional to the level of genomic gain or loss. Keywords: Rhabdomyosarcoma cell lines will be added later on

ORGANISM(S): Homo sapiens

SUBMITTER: Rogier Versteeg 

PROVIDER: E-GEOD-8840 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genomic imbalances in rhabdomyosarcoma cell lines affect expression of genes frequently altered in primary tumors: an approach to identify candidate genes involved in tumor development.

Missiaglia Edoardo E   Selfe Joanna J   Hamdi Mohamed M   Williamson Daniel D   Schaaf Gerben G   Fang Cheng C   Koster Jan J   Summersgill Brenda B   Messahel Boo B   Versteeg Rogier R   Pritchard-Jones Kathy K   Kool Marcel M   Shipley Janet J  

Genes, chromosomes & cancer 20090601 6


Rhabdomyosarcomas (RMS) are the most common pediatric soft tissue sarcomas. They resemble developing skeletal muscle and are histologically divided into two main subtypes; alveolar and embryonal RMS. Characteristic genomic aberrations, including the PAX3- and PAX7-FOXO1 fusion genes in alveolar cases, have led to increased understanding of their molecular biology. Here, we determined the effect of genomic copy number on gene expression levels through array comparative genomic hybridization (CGH)  ...[more]

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