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Transcription profiling of Xenopus eye primordia vs. posterior neural plate vs. lateral endoderm normalized the whole embryos

ABSTRACT: Tissues from the eye primordia, lateral endoderm, and posterior; neural plate of stage 15 Xenopus laevis embryos were isolated and normalized to stage 15 whole embryos. Three biological replicates were prepared for each tissue and the expression patterns were profiled using Affymetrix Xenopus Laevis GeneChip microarrays. Experiment Overall Design: Tissues from the eye primordia, lateral endoderm, and posterior Experiment Overall Design: neural plate of stage 15 Xenopus laevis embryos were isolated and normalized to stage 15 whole embryos. Three biological replicates were prepared for each tissue and the expression patterns were profiled using Affymetrix Xenopus Laevis GeneChip microarrays.

ORGANISM(S): Xenopus laevis

SUBMITTER: Michael Zuber 

PROVIDER: E-GEOD-9175 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Generation of functional eyes from pluripotent cells.

Viczian Andrea S AS   Solessio Eduardo C EC   Lyou Yung Y   Zuber Michael E ME  

PLoS biology 20090818 8

Pluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells. Animal pole cells isolated from blastula stage  ...[more]

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