Project description:Gene expression was measured on the Affymetrix platform in iPrEC cell line transfected with wild-type USP2A, mutant USP2A, or empty vector.
Project description:We have mapped regulatory loci for nearly all protein coding genes in the mammalian genome using microarray measurements from a panel of mouse/hamster radiation hybrids. Proof of principle transfection experiment. Pcdh7 (a gene identified as being a regulator of many genes) is transfected into A23 and HEK cells and compared to A23 and HEK cells transfected with an empty vector. Upregulated genes predicted by our approach are compared with the transfection experiments Experiment Overall Design: There are 4 microarrays. The first two are biological replicates of A23 (transfected with Pcdh7, labeled with cy5) / A23 (tranfected with empty vector, labeled with cy3). The second two are biological replicates of HEK (transfected with Pcdh7, labeled with cy5) / HEK (tranfected with empty vector, labeled with cy3).
Project description:We used microarrays to detail the global gene expression in human nasopharynx cell line transfected with gene or empty vector. human nasopharynx cell line transfected with gene or empty vector were for RNA extraction and hybridization on Affymetrix microarrays.
Project description:To study the effect of Notch 1 overexpression on cell funtion and to elucidate involved signalling pathways MLE12 cells tranfected with NICD1-pIRES-dsRed2 vector were analyzed for their transcript profiles 12, 24, and 48h after transfection and related to an empty vector (EV) transfected control cells
Project description:Gene expression was measured on the Affymetrix platform in iPrEC cell line transfected with wild-type USP2A, mutant USP2A, or empty vector. Gene expression was measured in 3 distinct groups (cell transfected with wild-type USP2A, mutant USP2A, or the empty vector). For each group 5 biological replicates were obtained and gene expression was measured on the Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. Differential gene expression was obtained for all the 3 possible pair-wise comparisons (WT versus MUT, WT versus EV, and MUT versus EV).
Project description:HeLa cells transfected with an empty vector or a TIPE1 lentiviral vector were collected for gene expression detection. Some of them, for instance, the expression of genes induced by p53 was dramatically decreased. We used microarrays to detail the gene expression after transfection with TIPE1 and identified distinct classes of changed genes during this process.