267B1, PC3M, LNCaP Methylation Profile vs. mRNA Expression Level
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Yipeng Wang
PROVIDER: E-GEOD-951 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Survey of differentially methylated promoters in prostate cancer cell lines.
Wang Yipeng Y Yu Qiuju Q Cho Ann H AH Rondeau Gaelle G Welsh John J Adamson Eileen E Mercola Dan D McClelland Michael M
Neoplasia (New York, N.Y.) 20050801 8
DNA methylation and copy number in the genomes of three immortalized prostate epithelial and five cancer cell lines (LNCaP, PC3, PC3M, PC3M-Pro4, and PC3M-LN4) were compared using a microarray-based technique. Genomic DNA is cut with a methylation-sensitive enzyme HpaII, followed by linker ligation, polymerase chain reaction (PCR) amplification, labeling, and hybridization to an array of promoter sequences. Only those parts of the genomic DNA that have unmethylated restriction sites within a few ...[more]
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