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Gene expression profiling in acute murine autoimmune hepatitis

ABSTRACT: The etiology of autoimmune hepatitis is poorly understood but likely involves Th1 cells producing IFN-γ. BALB/c background TGF-β1-/- mice rapidly develop fulminant Th1-mediated autoimmune hepatitis. Our aims are to profile liver gene expression in TGF-β1-/- mice, to identify gene expression pathways dependent on IFN-γ as possible targets for rational therapy, and to test potential targets directly in vivo in mice. Keywords: Comparative analysis of gene expression in livers of WT, TGFB1 & IFN knockout mice DNA microarray analyses were applied to liver RNA from TGF-β1-/- mice, TGF-β1-/- /IFN-γ-/- mice, and TGF-β1+/+ littermate controls. 3 mice from each group were analyzed in this study.

ORGANISM(S): Mus musculus

SUBMITTER: Jennifer Sargent

PROVIDER: E-GEOD-9892 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The role of Ifng in alterations in liver gene expression in a mouse model of fulminant autoimmune hepatitis.

Milks Michael W MW Cripps James G JG Lin Heping H Wang Jing J Robinson Richard T RT Sargent Jennifer L JL Whitfield Michael L ML Gorham James D JD

Liver international : official journal of the International Association for the Study of the Liver 20090416 9

<h4>Background/aims</h4>BALB/c mice with a homozygous deficiency in the Tgfb1 gene are a model of fulminant autoimmune hepatitis (AIH), spontaneously and rapidly developing Th1-mediated IFN-gamma-dependent necroinflammatory liver disease. We sought to understand the molecular basis for fulminant Th1 liver disease and the specific role of the Ifng gene.<h4>Methods</h4>Global gene expression in livers from BALB/c Tgfb1(-/-) mice with and without an intact Ifng gene was assessed by microarray analy ...[more]

PMID: 19490417

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Gene expression profiling in acute murine autoimmune hepatitis

Project description:The etiology of autoimmune hepatitis is poorly understood but likely involves Th1 cells producing IFN-γ. BALB/c background TGF-β1-/- mice rapidly develop fulminant Th1-mediated autoimmune hepatitis. Our aims are to profile liver gene expression in TGF-β1-/- mice, to identify gene expression pathways dependent on IFN-γ as possible targets for rational therapy, and to test potential targets directly in vivo in mice. Keywords: Comparative analysis of gene expression in livers of WT, TGFB1 & IFN knockout mice

2009-02-28 | GSE9892 | GEO

EAE_CD3_Sp/Br

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The transcription factor Bhlhe40 is a switch of inflammatory versus anti-inflammatory Th1 cell fate determination

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Real-time quantitative PCR analysis of human mononuclear cells from bone marrow.

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A novel non-canonical signaling pathway mediates TGF-β1-induced glucocorticoid insensitivity in epithelial cells

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Retinoic acid signaling constrains the plasticity of Th1 cells and prevents development of pathogenic Th17 cells [Affymetrix experiments]

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Real-time quantitative PCR analysis of human mononuclear cells from bone marrow.

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Project description:Activation of non-canonical TGF-β1 signaling indicates an autoimmune mechanism for bone marrow fibrosis in primary myelofibrosis

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