Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-sequencing and small RNA-sequencing of 465 lymphoblastoid cell lines from the 1000 Genomes

ABSTRACT: This RNA sequencing data set of 465 human lymphoblastoid cell line samples from the CEU, FIN, GBR, TSI and YRI populations from the 1000 Genomes sample collection was created by the Geuvadis consortium (www.geuvadis.org, http://www.geuvadis.org/web/geuvadis/our-rnaseq-project). The data is under embargo until the first publication by the investigators in early 2013. This accession contains all mRNA and small RNA sequencing data, clean data that passed QC and other filters are available under accession E-GEUV-1, E-GEUV-2.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Natalja Kurbatova

PROVIDER: E-GEUV-3 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Reproducibility of high-throughput mRNA and small RNA sequencing across laboratories.

't Hoen Peter A C PA Friedländer Marc R MR Almlöf Jonas J Sammeth Michael M Pulyakhina Irina I Anvar Seyed Yahya SY Laros Jeroen F J JF Buermans Henk P J HP Karlberg Olof O Brännvall Mathias M den Dunnen Johan T JT van Ommen Gert-Jan B GJ Gut Ivo G IG Guigó Roderic R Estivill Xavier X Syvänen Ann-Christine AC Dermitzakis Emmanouil T ET Lappalainen Tuuli T

Nature biotechnology 20130915 11

RNA sequencing is an increasingly popular technology for genome-wide analysis of transcript sequence and abundance. However, understanding of the sources of technical and interlaboratory variation is still limited. To address this, the GEUVADIS consortium sequenced mRNAs and small RNAs of lymphoblastoid cell lines of 465 individuals in seven sequencing centers, with a large number of replicates. The variation between laboratories appeared to be considerably smaller than the already limited biolo ...[more]

PMID: 24037425

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Dataset Information

RNA-sequencing and small RNA-sequencing of 465 lymphoblastoid cell lines from the 1000 Genomes

Publications

Reproducibility of high-throughput mRNA and small RNA sequencing across laboratories.

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