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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of S. coelicolor grown in liquid culture and heat shock stimulated

ABSTRACT: Global gene expression patterns of two biological replicate cultures of S.coelicolor MT1110 grown on YEME plus 10% sucrose up to early stationary phase ( the culture conditions were the same used for obtaining ChIP-chip data with anti HspR antibodies). RNA was harvested from heat-shocked cultures (42oC) and control cultures grown at 30oC and hybridized onto high density IJISS arrays to identify which genes are induced following heat shock in S. coelicolor

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Emma Laing

PROVIDER: E-MAXD-49 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon.

Bucca Giselda G Laing Emma E Mersinias Vassilis V Allenby Nicholas N Hurd Douglas D Holdstock Jolyon J Brenner Volker V Harrison Marcus M Smith Colin P CP

Genome biology 20090116 1

<h4>Background</h4>DNA microarrays are a key resource for global analysis of genome content, gene expression and the distribution of transcription factor binding sites. We describe the development and application of versatile high density ink-jet in situ-synthesized DNA arrays for the G+C rich bacterium Streptomyces coelicolor. High G+C content DNA probes often perform poorly on arrays, yielding either weak hybridization or non-specific signals. Thus, more than one million 60-mer oligonucleotide ...[more]

PMID: 19146703

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