Global distribution of RNA polymerase in Streptomyces coelicolor
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ABSTRACT: Genome wide ChIP-chip analysis of RNA Polymerase binding sites in Streptomyces coelicolor MT1110 cultures grown in SMM + 0.5% casaminoacids at 30C up to mid exponential and stationary phase. Chromatin extracted from rifampicin and formaldehyde treated cultures was incubated with anti ? subunit of core RNA polymerase of E.coli monoclonal antibodies and the DNA purified from anti RNA Polymerase IP and mock IP samples was labelled with either Cy3 or Cy5- dCTP and hybridized in an direct type of microarray experiment onto SCO-Chip2-v2 High Density IJISS arrays
ORGANISM(S): Streptomyces coelicolor
SUBMITTER: Emma Laing
PROVIDER: E-MAXD-60 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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