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Unknown,Transcriptomics,Genomics,Proteomics

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ChIP on chip data for AbrC3 binding sites in S. coelicolor M145

ABSTRACT: ChIP on chip assay of AbrC3 to determine its binding sites in S. coelicolor M145 genome using purified anti-AbrC3 polyclonal antibodies. Samples from nutrient broth (NB) cultures were collected at 36, 48 and 60 hours, cross-linked, and finally hybridized to DNA 104K microarrays designed by University of Surrey.

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Emma Laing

PROVIDER: E-MTAB-1451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deciphering the regulon of Streptomyces coelicolor AbrC3, a positive response regulator of antibiotic production.

Rico Sergio S Santamaría Ramón I RI Yepes Ana A Rodríguez Héctor H Laing Emma E Bucca Giselda G Smith Colin P CP Díaz Margarita M

Applied and environmental microbiology 20140207 8

The atypical two-component system (TCS) AbrC1/C2/C3 (encoded by SCO4598, SCO4597, and SCO4596), comprising two histidine kinases (HKs) and a response regulator (RR), is crucial for antibiotic production in Streptomyces coelicolor and for morphological differentiation under certain nutritional conditions. In this study, we demonstrate that deletion of the RR-encoding gene, abrC3 (SCO4596), results in a dramatic decrease in actinorhodin (ACT) and undecylprodiginine (RED) production and delays morp ...[more]

PMID: 24509929

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