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Transcription profiling of wild type and C-hordein knock-out barley line

ABSTRACT: Identify the transcriptional effects of the antisense C-hordein construct in a barley line

INSTRUMENT(S): arrayWoRx Biochip Reader [Applied Precision]

ORGANISM(S): Hordeum vulgare

SUBMITTER: Michael Hansen

PROVIDER: E-MEXP-1000 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Antisense-mediated suppression of C-hordein biosynthesis in the barley grain results in correlated changes in the transcriptome, protein profile, and amino acid composition.

Hansen Michael M Lange Mette M Friis Carsten C Dionisio Giuseppe G Holm Preben Bach PB Vincze Eva E

Journal of experimental botany 20070101 14

Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marke ...[more]

PMID: 18162630

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