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Transcription profiling of two human colon cancer cell lines treated with n-3 PUFA docosahexaenoic acid for 3 different time points

ABSTRACT: Several studies indicate that omega (n)-3 fatty acids may have a potential in the prevention and treatment of cancer. In this study the colon cancer cell lines SW480 and SW620 were treated with the n-3 PUFA DHA (Docosahexaenoic acid) for 3 different time points (12, 24 and 48h). The experiments were performed in three independent replicates (1-3), RNA isolated and used for Affymetrix gene expression analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Caroline Jakobsen

PROVIDER: E-MEXP-1014 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis.

Jakobsen Caroline Hild CH Størvold Gro Leite GL Bremseth Hilde H Follestad Turid T Sand Kristin K Mack Merete M Olsen Karina Standahl KS Lundemo Anne Gøril AG Iversen Jens Gustav JG Krokan Hans Einar HE Schønberg Svanhild Arentz SA

Journal of lipid research 20080619 10

Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphor ...[more]

PMID: 18566476

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Transcription profiling of human colon cancer cell line SW620 treated with docosahexaenoic acid

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2009-06-01 | E-MEXP-2010 | biostudies-arrayexpress

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Transcription profiling of human human neuroblastoma SH-SY5Y cells identifies molecular effectors of unconjugated bilirubin

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2009-12-02 | E-GEOD-16768 | biostudies-arrayexpress

Gene expression profile in response to doxorubicin-rapamycin combined treatment of HER-2 overexpressing human mammary epithelial cell lines

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Effects of redox modulation by inhibition of Thioredoxin reductase on radiosensitivity and gene expression

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2005-09-01 | E-MEXP-377 | biostudies-arrayexpress

Transcription profiling of rat chondrocytes treated with glucosamine exogenous glucosamine globally protects chondrocytes from the arthritogenic effects of IL-1beta

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2009-04-23 | E-GEOD-6119 | biostudies-arrayexpress

Transcription profiling of human neuroblastoma SH-SY5Y cells: 4h identifies molecular effectors of unconjugated bilirubin

Project description:The deposition of unconjugated bilirubin (UCB) in selected regions of the brain results in irreversible neuronal damage, or Bilirubin Encephalopathy (BE). Although UCB impairs a large number of cellular functions, the basic mechanisms of neurotoxicity have not yet been fully clarified. While cells can accumulate UCB by passive diffusion, cell protection may involve multiple mechanisms including the extrusion of the pigment as well as pro-survival homeostatic responses that are still unknown. The effects of UCB treatment to SH-SY5Y neuroblastoma cell line were examined by high-density oligonucleotide microarrays. 230 genes were induced after 24 hours. A Gene Ontology (GO) analysis showed that a large group of UCB-induced genes were components of the ER stress response. Independent experimental validation of molecular events crucial for the ER stress response is presented. The results show that UCB exposure induces the ER stress response as a major intracellular homeostatic response in neuroblastoma cells in vitro. Our finding may provide valuable information for new therapeutic strategies in the treatment of BE. Experiment Overall Design: We used high-density oligonucleotide microarrays to analyze the gene expression profile of human neuroblastoma SH-SY5Y cells upon UCB treatment. Three replicates of UCB-treated cells were analyzed. As controls, we used three replicates of the same cells treated with DMSO only (the solvent used for UCB treatment).

2009-12-02 | E-GEOD-16767 | biostudies-arrayexpress

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2009-11-29 | E-GEOD-16656 | biostudies-arrayexpress

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