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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MicroRNA profiling of wild type and p72 knock-out mice embryos

ABSTRACT: To examine the effect of DEAD-box RNA helicase subunits on the primary miRNAs processing by Drosha complex, we made knockout mice of p72, DEAD-box RNA helicase, a component of Drosha complex. And we compare the miRNA expression profiles derived from whole mice embryo between wild-type and p72 KO mice.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Mus musculus

SUBMITTER: KAORU YAMAGATA

PROVIDER: E-MEXP-1052 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DEAD-box RNA helicase subunits of the Drosha complex are required for processing of rRNA and a subset of microRNAs.

Fukuda Toru T Yamagata Kaoru K Fujiyama Sally S Matsumoto Takahiro T Koshida Iori I Yoshimura Kimihiro K Mihara Masatomo M Naitou Masanori M Endoh Hideki H Nakamura Takashi T Akimoto Chihiro C Yamamoto Yoko Y Katagiri Takenobu T Foulds Charles C Takezawa Shinichiro S Kitagawa Hirochika H Takeyama Ken-ichi K O'Malley Bert W BW Kato Shigeaki S

Nature cell biology 20070415 5

MicroRNAs (miRNAs) control cell proliferation, differentiation and fate through modulation of gene expression by partially base-pairing with target mRNA sequences. Drosha is an RNase III enzyme that is the catalytic subunit of a large complex that cleaves pri-miRNAs with distinct structures into pre-miRNAs. Here, we show that both the p68 and p72 DEAD-box RNA helicase subunits in the mouse Drosha complex are indispensable for survival in mice, and both are required for primary miRNA and rRNA pro ...[more]

PMID: 17435748

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