Project description:We compared gene expression patterns between the occipital cortex tissues of four male and four female individuals in three species: an ape (human, Homo sapiens), an Old World monkey (macaque; Macaca fascicularis), and a New World monkey (marmoset; Callithrix jacchus). To do so, we hybridized cDNA from each sample (n = 24) to a human cDNA microarray that contains 46,128 probes. (Human 46k cDNA, http://www.biotech.kth.se/molbio/microarray/). We used a loop hybridization study design restricted to within-species comparisons only, in which we co-hybridized on each slide samples from the opposite sex.
Project description:Multiple studies have shown that brain gene expression is disturbed in subjects suffering from schizophrenia. However, disentangling disease effects from alterations caused by neuroleptics is a challenging task. <br> To address the issues of disease heterogeneity and the effect of antipsychotic medication, we use a large collection of 110 brain autopsy samples, subdivided according to the type of antipsychotic medication received. We use global and high-resolution mRNA quantification techniques to analyse gene expression in brain tissues. We also analyse transcription in-vitro, in cell lines before and after treatment with cytokines.<br> Results. We show that inflammation-related genes are up regulated in all groups of patients, including those not treated at the time of death. In particular, four co-expressed genes that are inducible by inflammatory cytokines showed increased mRNA levels, namely IFITM2, IFITM3, SERPINA3, and GBP1 (p-values from qPCR ? 0.01). We also show that these genes are expressed in oligodendrocyte and endothelial cell lines, and transcription is inducible by inflammatory cytokines in both cell lines. Our results give molecular support to the inflammatory theory of schizophrenia and suggest that inflammatory cytokines may stimulate transcription in endothelial brain cells during schizophrenia progression. Unexpectedly, inflammation-related genes may also have a role in myelin producing cells, since they are also inducible by cytokines in oligodendrocyte cell lines. Our findings encourage future research to explore whether anti-inflammatory agents can be used in combination with traditional antipsychotics for a more efficient treatment of schizophrenia.
Project description:We studied the effect of small interfering RNA (siRNA)-mediated QKI depletion on global gene expression in human oligodendroglioma cells and astrocyte glioma cells
Project description:Gerbera petals of inflorescence developmental stages 2, 4, 6, 7, 8, and 9 were compared against a reference sample that included same amount of mRNA from stages 1-11. Alltogether six comparisons were done each including two biological replicates with 3 technical replicates resulting alltogether 36 hybridizations. At least one of each biological replicate was dyeswap hybridization. This experiment was done in order to profile transcriptional changes that occur in petals from stage where they still divide until senescence of the cells. Developmental stages have been characterized by Helariutta et al. (1995).
Project description:Gerbera inflorescence sample was compared with a gerbera leaf sample in order to identify flower specific genes. Samples were pooled from different developmental stages and 4 replicants were independently labelled. All labellings/hybridizations included two dye swap replicants. Gerbera 9K cDNA microarrays were used for the hybridizations.
Project description:Gerbera inflorescence sample was compared with a gerbera leaf sample in order to identify flower specific genes. Samples were pooled from different developmental stages and alltogether 8 replicates (including 2 biological replicates with 4 technical replicates each) were independently labelled. Both biological replicates included two dye swap replicates. Gerbera 9K cDNA microarrays were used for the hybridizations.
Project description:Two different strains of Aedes aegypti mosquito, Moyo-in-dry and Moyo-S, are profiled for their response through time to infection with Dengue 2 virus. Expression is measured using a two-colour custom spotted cDNA array. A mixed strain uninfected sample is hybridized as the reference.
Project description:Evaluation and comparison of biological responses of rats and gene expression responses of the kidney in rats exposed to different doses of Puromycin [CAS:53-79-2;CHEBI:17939]