Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or CEN4-ARS1-URA3 yeast sequences

ABSTRACT: Influence of a wild type DNA Topoisomerase IB on global transcription profiles in exponentially growing S. cerecisiae cells in SC medium plus 2% of glucose.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Giovanni Capranico

PROVIDER: E-MEXP-1080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or an inactive Y727F mutant enzyme and treated with camptothecin

Project description:To establish the effect of CPT in the regulation of global transcription in S. cerevisiae, we have used a yeast TOP1 null strain, JEL1Δtop1, bearing a low-copy number plasmid that expresses, under the control of the yeast TOP1 promoter, either a wild-type (wt) yeast Top1p (pCC10) or an inactive Y727F mutant enzyme (pAR7).We determined global transcript levels upon CPT treatments of JEL1Δtop1 cells expressing either a yeast wt or Y727F mutant Top1p.

2008-06-01 | E-MEXP-1104 | biostudies-arrayexpress

Transcription profiling of hearts from young (2-4 month) and moderately aged (16-18 month) C57/Bl6 male mice subjected to i) 20 min global ischaemia, 60 min reperfusion or ii) 80 min aerobic perfusion/normoxia

Project description:Hearts from young (2-4 month) and moderately aged (16-18 month) C57/Bl6 male mice were subjected to i) 20 min global ischaemia, 60 min reperfusion or ii) 80 min aerobic perfusion/normoxia.

2005-12-31 | E-MEXP-496 | biostudies-arrayexpress

Transcription profiling of mouse aorta (whole or regions) from mice of different embryonic ages

Project description:In order to identify novel regulators of haematopoietic stem cell emergence in the mouse embryonic AGM region, 3 different types of gene expression comparisons were performed. In the first one, whole dorsal aortas from embryonic day (E) 11 embryos and E9 embryos were dissected, tissues from each time point were pooled and their expression profile compared (whole aorta (WA) E9 vs E11). 3 different pools/biological replicas were obtained for each time point, a dye swap was included in each experiment, a technical replicate was performed for each pool (including the dye swap) and 2 self-self hybridisation controls were included. In the second comparison, GFP+ cells were isolated by fluorescence-activated cell sorting from pooled E9 and E11 aortas from transgenic embryos that express GFP under the regulatory elements of the Ly-6A gene. We obtained one pool for each time point and compared their transcription profile (GFP E9 vs E11). This comparison also contained a dye swap and a technical replicate (also of the dye swap). In the third comparison, the E11 aorta was cut into 3 roughly equal parts. Pools were obtained of the middle region ("m") and the two outer regions, rostral and caudal, together ("r+c") and their expression profile compared (11AO m vs r+c). There was one pool of each population, and the experiment included a dye swap and a technical replicate (also of the dye swap) and one self-self hybridisation control. Isolated RNA was amplified by T7-mediated in vitro transcription, with one round for the first and the third comparison and two rounds for the second comparison (sorted GFP+ cells). Probes were labelled during the reverse transcription step. Within array normalisation was achieved with Lowess Smooth and between arrays with Z scores. ANOVA analysis identified differentially expressed genes and K means clustering was used to segregate them into upregulated and downregulated genes.

2009-06-06 | E-TABM-659 | biostudies-arrayexpress

Transcription profiling of Arabidopsis len3 mutants

Project description:Arabidopsis lesion initiation 3 mutant

2008-04-17 | E-MEXP-1084 | biostudies-arrayexpress

Comparative genomic hybridization of 5 human prostate cancer cell lines and 13 prostate cancer xenografts to create genomic profiles of copy number alterations

Project description:Array-CGH was done with 5 prostate cancer cell lines and 13 prostate cancer xenografts to create genomic profiles of copy number alterations. The profiles were used to identify common alterations and define minimal regions of copy number changes. A normal female to normal male hybridisation was done to check the experimental conditions.

2005-12-31 | E-MEXP-335 | biostudies-arrayexpress

Transcription profiling of human dermal microvascular endothelial, fibroblast, and glioblastoma cells treated with mycophenolic acid

Project description:The effect of Mycophenolic acid on primary isolated human dermal microvascular endothelial (HDMVEC) and fibroblast cells as well as human glioblastoma brain tumor cell line (U87).

2008-06-25 | E-TABM-315 | biostudies-arrayexpress

Transcription profiling of Arabidopsis overexpressing DREB2Aca vs wild type plants

Project description:Constitutive active form of DREB2A, a transcriptional regulator involving to plant drought and high-salinity stress response, was overexpressed in Arabidopsis, and gene expression pattern was compared between transgenic plants and wild type plants.

2006-04-01 | E-MEXP-317 | biostudies-arrayexpress

Transcription profiling of S. pombe wild type and meu5delta cells during pat1 induced meiosis, wild type meiosis and overexpression of the mei4 transcription factor

Project description:The Meu5 RNA-binding protein stabilises its targets during meiosis. We compared wild type and meu5delta cells transcriptome under different conditions: pat1-induced meiosis in diploid cells, wild type meiosis in diploid cells and cells overexpressing the Mei4 transcription factor.

2011-01-01 | E-TABM-775 | biostudies-arrayexpress

Transcription profiling of S. pombe wild type and meu5 deletion mutants during meiosis

Project description:The Meu5 RNA-binding protein stabilises its targets during meiosis. To investigate the function of Meu5 we induced synchronous meiosis in wild type or meu5delta cells (using pat1 thermo-sensitive mutations), and measured mRNA levels at regular intervals.

2011-01-01 | E-TABM-774 | biostudies-arrayexpress

Transcription profiling time series of human HeLa cells cultivated while sterol depleted from the medium

Project description:The aim of the experiment was to identify novel genes with relevance to cellular cholesterol metabolism. HeLa cells were cultivated while sterol was depleted from the medium by applying 2-Hydroxypropyl-cyclodextrin. RNA for expression profiling was isolated 3h, 4.5h and 6h after depletion.

2008-12-17 | E-TABM-599 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data