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Transcription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or an inactive Y727F mutant enzyme and treated with camptothecin

ABSTRACT: To establish the effect of CPT in the regulation of global transcription in S. cerevisiae, we have used a yeast TOP1 null strain, JEL1Δtop1, bearing a low-copy number plasmid that expresses, under the control of the yeast TOP1 promoter, either a wild-type (wt) yeast Top1p (pCC10) or an inactive Y727F mutant enzyme (pAR7).We determined global transcript levels upon CPT treatments of JEL1Δtop1 cells expressing either a yeast wt or Y727F mutant Top1p.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Giovanni Capranico

PROVIDER: E-MEXP-1104 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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