Transcription profiling of Zea mays leaves from plants grown under normal or enriched carbon dioxide conditions

ABSTRACT: Zea may L. hybrid H99 plants were grown for eight weeks in compost (Driscoll et al., 2006) in duplicate controlled environment cabinets (Sanyo 970, SANYO, Osaka) where the atmospheric CO2 was maintained at either 350 uL L-1 or at 700 uL L-1. The plants were exposed to 16 h photoperiod (700 ¥ìmol m-2 s-1) and the temperature was maintained at 25¨¬C (day) and 19¨¬C (night) with 80% relative humidity. The CO2 was supplied from a bulk container, transmitted via a Vaisala GMT220 CO2 transmitter (VAISALA OYJ, Helsinki, Finland), and maintained by a Eurotherm 2704 controller (EUROTHERM LTD., Worthing, U.K.) which kept CO2 levels at 350 ¡¾ 20 uL L-1 or 700 ¡¾ 20 uL L-1. All plants were watered everyday throughout development in order to avoid a water stress, especially in plants grown at 700 uL L-1. Total RNA was extracted using Trizol reagent (Invitrogen, UK). For the microarray study, leaf 12 (young leaf) and leaf 3 (senescent leaf) of 8 week-old maize plants grown at 350uL L-1 or 700uL L-1 CO2 were used. Eight plants from each treatment were sampled, with equal amounts of RNA from 2-3 plants being pooled to obtain 3 replicate samples. Preparation of RNA and microarray hybridisation and chip processing was performed at AROSab, Denmark.

ORGANISM(S): Zea mays

SUBMITTER: Anneke Prins 

PROVIDER: E-MEXP-1222 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress