Transcription profiling of Lotus japonicus grown under different nitrogen sources
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ABSTRACT: Transcriptomic profiling was carried out for leaves of Lotus japonicus plants grown with different mineral nitrogen sources (NO3-, NH4+ or NH4NO3) or under conditions of biological nitrogen fixation (Nod). Nodulated plants were inoculated with Mesorhizobium loti and watered with nitrogen-free âHornumâ medium supplemented with 3 mM KCl. Plants under different nitrogen nutritions were watered with âHornumâ nutrient solution containing 10 mM KNO3 (NO3- plants) or with 10 mM NH4Cl supplemented with 3 mM KCl (NH4+ plants) or with 5 mM NH4NO3 supplemented with 3 mM KNO3 (NH4NO3 plants). After all the plants reached the size of 7 trifoils, leaf tissue was harvested. Every harvest involved at least three independent biological replicates for each treatment.
ORGANISM(S): Lotus japonicus
SUBMITTER: Carmen MarÃa Pérez Delgado de Torres
PROVIDER: E-MTAB-4177 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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