Transcription profiling of human HepG2 cells expressing gain-of-function mutant D374Y-PCSK9
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Morten Mattingsdal
PROVIDER: E-MEXP-1235 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Genome-wide expression analysis of cells expressing gain of function mutant D374Y-PCSK9.
Ranheim Trine T Mattingsdal Morten M Lindvall Jessica M JM Holla Oystein L OL Berge Knut Erik KE Kulseth Mari Ann MA Leren Trond P TP
Journal of cellular physiology 20081101 2
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a key regulator of serum cholesterol. The possibility that PCSK9 also functions in other pathways needs to be addressed. We have transfected HepG2 cells with mutant D374Y-PCSK9 or control vector. Gene expression signatures were determined using the Affymetrix GeneChip technology, and the expression pattern of selected genes was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Data was normalized and analyzed using a ...[more]
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