Transcription profiling by array of prostate from mice at different stages of JOCK1 prostate cancer progression to identify FGFR1-mediated gene expression changes

ABSTRACT: FGFR1 mediated changes in gene expression at different stages of JOCK1 prostate cancer progression JOCK1 mice which express a prostate localize inducible version of the FGFR1 gene (iFGFR1) were used to determine what are the changes in gene expression at distinct stages of JOCK1 tumor progression. Activation of iFGFR1 is mediated by biweekly treatements of JOCK1 mice with AP20187 (50mg/kg). Wild type mice were treated for 24 hours with CID as a control, while JOCK1 were treated with CID for 4, 12, 24 and 52 weeks. All these treatments began at 12 weeks of age. After treatment mouse prostates (ventral and dorso-lateral prostate lobes), were dissected, placed in RNAlater and incubated at -800C. Tissue was thawed and placed in TRIzol (Invitrogen) for RNA isolation according to the manufacture. Once RNA was removed, a RNA cleaning step was performed using Qiagen RNEasy columns as described by the manufacturer. Time points were performed in triplicate and each array represents a single prostate. Labeling and hybridization to the mouse Affymetrix gene array 430 2.0 was performed by the Baylor College of Medicine microarray core. All hybridizations were performed at the same time.

ORGANISM(S): Mus musculus

DISEASE(S): prostate cancer

SUBMITTER: Victor Acevedo 

PROVIDER: E-MEXP-1296 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress