Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of tissues of Arabidopsis plants overexpressing artificial microRNAs vs wild_type and respective target gene mutants


ABSTRACT: Tissues of Arabidopsis plants overexpressing artificial microRNAs were compared to wild_type and respective target gene mutants (duplicate arrays)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Stephan Ossowski 

PROVIDER: E-TABM-63 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Highly specific gene silencing by artificial microRNAs in Arabidopsis.

Schwab Rebecca R   Ossowski Stephan S   Riester Markus M   Warthmann Norman N   Weigel Detlef D  

The Plant cell 20060310 5


Plant microRNAs (miRNAs) affect only a small number of targets with high sequence complementarity, while animal miRNAs usually have hundreds of targets with limited complementarity. We used artificial miRNAs (amiRNAs) to determine whether the narrow action spectrum of natural plant miRNAs reflects only intrinsic properties of the plant miRNA machinery or whether it is also due to past selection against natural miRNAs with broader specificity. amiRNAs were designed to target individual genes or g  ...[more]

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