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Chromatin immunopreciptiation of human breast epithelial HME1 cell lines over-expressing wild type or mutant p53 to study p53 binding

ABSTRACT: p53 binding was studied using ChIP on chip technique in breast epithelial HME1 cell lines overexpressing wt or mutant p53

ORGANISM(S): Homo sapiens

SUBMITTER: Lukas Vrba

PROVIDER: E-MEXP-1491 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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p53 induces distinct epigenetic states at its direct target promoters.

Vrba Lukas L Junk Damian J DJ Novak Petr P Futscher Bernard W BW

BMC genomics 20081015

<h4>Background</h4>The tumor suppressor protein p53 is a transcription factor that is mutated in many cancers. Regulation of gene expression by binding of wild-type p53 to its target sites is accompanied by changes in epigenetic marks like histone acetylation. We studied DNA binding and epigenetic changes induced by wild-type and mutant p53 in non-malignant hTERT-immortalized human mammary epithelial cells overexpressing either wild-type p53 or one of four p53 mutants (R175H, R249S, R273H and R2 ...[more]

PMID: 18922183

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