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Chromatin immunoprecipitation of trimethylated histone H3-K27 in human prostate cancer cell line PC3

ABSTRACT: Epigenetic silencing in cancer cells is mediated by at least two distinct histone modifications, polycomb based histone H3 lysine 27 trimethylation (H3K27triM) and H3K9 dimethylation.

Using chromatin immunoprecipitation-microarrays (ChIP-chip) in prostate cancer cells, we found that up to 5% of promoters (16% CpG islands and 84% non-CpG islands) were enriched with H3K27triM. These genes were silenced specifically in prostate cancer, and those CpG islands affected showed low levels of DNA methylation.

ORGANISM(S): Homo sapiens

DISEASE(S): prostate cancer

SUBMITTER: Yutaka Kondo

PROVIDER: E-MEXP-1585 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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