Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

32 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-chip by array of mouse 416Bcell line using anti-PU.1 (Santa Cruz, sc-352X).

ABSTRACT: Immunoprecipitations were performed using anti-PU.1 (Santa Cruz, sc-352X)

ORGANISM(S): Mus musculus

SUBMITTER: Fernando Calero-Nieto

PROVIDER: E-MEXP-2666 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Transcriptional regulation of Elf-1: locus-wide analysis reveals four distinct promoters, a tissue-specific enhancer, control by PU.1 and the importance of Elf-1 downregulation for erythroid maturation.

Calero-Nieto Fernando J FJ Wood Andrew D AD Wilson Nicola K NK Kinston Sarah S Landry Josette-Renée JR Göttgens Berthold B

Nucleic acids research 20100604 19

Ets transcription factors play important roles during the development and maintenance of the haematopoietic system. One such factor, Elf-1 (E74-like factor 1) controls the expression of multiple essential haematopoietic regulators including Scl/Tal1, Lmo2 and PU.1. However, to integrate Elf-1 into the wider regulatory hierarchies controlling haematopoietic development and differentiation, regulatory elements as well as upstream regulators of Elf-1 need to be identified. Here, we have used locus- ...[more]

PMID: 20525788

Similar Datasets

ChIP-chip by array of mouse cell lines and fresh tissue using anti-acetyl histone H3 antibody.

Project description:Immunoprecipitations were performed using anti-acetyl histone H3 antibody (Millipore, 06-599)

2011-04-07 | E-MEXP-2664 | biostudies-arrayexpress

ChIP-chip by array of mouse cell lines and fresh tissues using anti-H3 trimethyl lysine 4.

Project description:Immunoprecipitations were performed using anti-H3 trimethyl lysine 4 (Millipore, 04-745 and gift from Dr. L. OM-oM->M-^RNeill, Birmingham, UK)

2010-06-09 | E-MEXP-2665 | biostudies-arrayexpress

ChIP-chip by array of mouse haematopoietic and endothelial cell lines using an anti-H3 trimethyl lysine 4 antibody

Project description:chromatin immunoprecipitations were performed in mouse haematopoietic and endothelial cell lines using an anti-H3 trimethyl lysine 4 antibody

2011-09-06 | E-MEXP-2908 | biostudies-arrayexpress

ChIP-chip by array of human T-acute lymphoblastic leukaemia cells using AcH3 antibodies

Project description:T-ALL Xenograft Histone Modification at ERG locus: The cells used are human T-acute lymphoblastic leukaemia cells that have been propagated in NOD/SCID mice. T-ALL8,T-ALL16, T-ALL27, T-ALL29, T-ALL30 and T-ALL31 are cells from six different patients. Normalized data files containing chromosome 21 data are available on the FTP site for this experiment in the E-MTAB-431.additional.zip archive.

2012-01-01 | E-MTAB-431 | biostudies-arrayexpress

ChIP-chip by array of human B-acute lymphoblastic leukaemia cells using AcH3, H3K4M3 and H3K27M3 antibodies

Project description:B-ALL Xenograft Histone Modification at BIM locus The cells used are human B- acute lymphoblastic leukaemia cells that have been propagated in NOD/SCID mice. B-ALL2 and B-ALL3 are cells from two different patients. Normalized data files containing chromosome 2 data are available on the FTP site for this experiment in the E-MEXP-2814.additional.zip archive.

2011-06-14 | E-MEXP-2814 | biostudies-arrayexpress

Chromatin immunoprecipitation of trimethylated histone H3-K27 in human prostate cancer cell line PC3

Project description:Epigenetic silencing in cancer cells is mediated by at least two distinct histone modifications, polycomb based histone H3 lysine 27 trimethylation (H3K27triM) and H3K9 dimethylation. Using chromatin immunoprecipitation-microarrays (ChIP-chip) in prostate cancer cells, we found that up to 5% of promoters (16% CpG islands and 84% non-CpG islands) were enriched with H3K27triM. These genes were silenced specifically in prostate cancer, and those CpG islands affected showed low levels of DNA methylation.

2008-04-26 | E-MEXP-1585 | biostudies-arrayexpress

Chromatin immunoprecipitation of wild type yeast using anti-H3C and anti-pol II antibodies

Project description:Chromatin immunoprecipitation were performed in yeast WT cells using anti-H3C and anti-pol II antibodies

2008-12-12 | E-MEXP-1951 | biostudies-arrayexpress

Transcription profiling by array of human universal RNA spiked with eight arifical transcripts at different copy numbers

Project description:Examine the dose response characteristics of a set of eight artificial transcripts (IVT) when spiked into a Universal Human RNA background at different copy numbers. The experiment was designed to (i) evaluate the technical reproducibility of array data generated on the Agilent Whole Human 44K array with use of a set of external RNA controls (ii) employ two panels of the same external RNA controls, which differed in copy number between panels, in order to simulate normal and disease states. We employed 8 different pools which were comprised of 8 different external RNA standards in a total human reference RNA background. Each different standard was present at a different copy number within any single pool (see file: E-MEXP-2670.additional.zip). Each control was present at a different concentration in each of the 8 pools. Standard microarray analysis was performed on the data and the differences in abundance between external RNA controls determined in terms of fold change differences.

2011-03-30 | E-MEXP-2670 | biostudies-arrayexpress

Comparative genomic hybridization of Lactobacillus sakei strain 23K and non-sequenced strains

Project description:CGH of L. sakei strain 23K vs non-sequenced strains

2009-09-01 | E-MEXP-1639 | biostudies-arrayexpress

Comparative genomic hybridization of Lactobacillus sakei strains

Project description:CGH of L. sakei strains from various origins.

2009-12-17 | E-MEXP-1969 | biostudies-arrayexpress