Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of adipose tissue from mesenteric and subcutaneous depots from 11beta-HSD1 knockout and wild type mice

ABSTRACT: Adipose tissue from mesenteric and subcutaneous depots from 11beta-HSD1 knockout (KO) and wild-type (WY) mice. Five biological replicates in each group: mesenteric KO, mesenteric WT, subcutaneous KO, subcutaneous WT.

ORGANISM(S): Mus musculus

SUBMITTER: Donald Dunbar

PROVIDER: E-MEXP-1636 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Novel fat depot-specific mechanisms underlie resistance to visceral obesity and inflammation in 11 β-hydroxysteroid dehydrogenase type 1-deficient mice.

Wamil Malgorzata M Battle Jenny H JH Turban Sophie S Kipari Tiina T Seguret David D de Sousa Peixoto Ricardo R Nelson Yvonne B YB Nowakowska Dominika D Ferenbach David D Ramage Lynne L Chapman Karen E KE Hughes Jeremy J Dunbar Donald R DR Seckl Jonathan R JR Morton Nicholas M NM

Diabetes 20110224 4

<h4>Objective</h4>The study objective was to determine the key early mechanisms underlying the beneficial redistribution, function, and inflammatory profile of adipose tissue in 11β-hydroxysteroid dehydrogenase type 1 knockout (11β-HSD1(-/-)) mice fed a high-fat (HF) diet.<h4>Research design and methods</h4>By focusing on the earliest divergence in visceral adiposity, subcutaneous and visceral fat depots from 11β-HSD1(-/-) and C57Bl/6J control mice fed an HF diet for 4 weeks were used for compar ...[more]

PMID: 21350084

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Project description:*Background: Adipocytes mainly function as energy storage and endocrine cells. The amount and distribution of fat are important factor that influence the meat quality in the beef industry. Fat depot can be found around internal organ (ometal), beneath the skin (subcutaneous), and between muscles (intramuscular). Different adipose depot showed the biological and genetic difference depending on their location. This inter-depot variation might be influenced by the inherent genetic programing for development of adipose depots. In this study, we used RNA-seq data to investigate the difference in transcriptome of various adipose depots in Hanwoo. *Results: Using RNA-seq, we identified 5797, 2156, and 5455 DEGs in the comparison between OI, OS, and IS respectively (FDR<0.01) and found 853, 48, and 979 DEGs specific to subcutaneous, intramuscular and omental fat respectively. DEGs in intramuscular fat were highly enriched the metabolism related pathways compared to other fat depots. DEGs specific to the omental fat is significantly enriched in PPAR signaling pathway and cell-junction related pathway. In subcutaneous fat, cytokine-cytokine receptor interaction with chemokines (CXC and CC subfamily) was the most significantly enriched the pathways. Interestingly, melanogenesis pathway was associated with the subcutaneous depot. Even though the adipose tissues shared the same pathways for adipocyte differentiation, the regulation of genes were different based on the depot. *Conclusions: We comparatively analyzed the transcripome profile from different adipose tissues using NGS and identified DEGs between adipose depot and specific to depot in Hanwoo animals. The functional annotation analysis of DEGs found that transcriptome profile difference in various adipose tissue of intramuscular, subcutaneous, and ometal fat. whole mRNA sequencing profiles of nine Korean native cattle (nine profiles of omental fat tissue, nine profiles of intramuscular fat tissue, nine profiles of subcutaneous fat tissue and eight profiles of muscle tissue)

Dataset Information

Transcription profiling of adipose tissue from mesenteric and subcutaneous depots from 11beta-HSD1 knockout and wild type mice

Publications

Novel fat depot-specific mechanisms underlie resistance to visceral obesity and inflammation in 11 β-hydroxysteroid dehydrogenase type 1-deficient mice.

Similar Datasets

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