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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of liver-specific Hnf4alpha knockout and wild type mouse liver

ABSTRACT: Gene expression differences between wild-type and liver-specific Hnf4alpha knockout (Albumin Cre+/-; Hnf4 fl/fl) mouse liver were assessed using RNA obtained from 2-month-old control and genetically modified female mice. RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].

ORGANISM(S): Mus musculus

SUBMITTER: Joan-Marc Servitja

PROVIDER: E-MEXP-1733 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional targets of the monogenic diabetes transcription factors HNF-1alpha and HNF-4alpha are highly conserved between mice and humans.

Boj Sylvia F SF Servitja Joan Marc JM Martin David D Rios Martin M Talianidis Iannis I Guigo Roderic R Ferrer Jorge J

Diabetes 20090202 5

<h4>Objective</h4>The evolutionary conservation of transcriptional mechanisms has been widely exploited to understand human biology and disease. Recent findings, however, unexpectedly showed that the transcriptional regulators hepatocyte nuclear factor (HNF)-1alpha and -4alpha rarely bind to the same genes in mice and humans, leading to the proposal that tissue-specific transcriptional regulation has undergone extensive divergence in the two species. Such observations have major implications for ...[more]

PMID: 19188435

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Transcription profiling of pancreatic islets from wild type, pancreas-specific Hnf4alpha knockout, heterozygous Hnf1alpha, and double mutant mice

Project description:Gene expression differences between wild-type, pancreas-specific Hnf4alpha knockout (Hnf4a-pKO), heterozygous Hnf1alpha (Tcf1) (Hnf1aHET), and double mutant (Hnf4a-pKO;Hnf1aHET) mouse pancreatic islets were assesed using amplified RNA samples from islets of matched quality and endocrine purity, obtained from 60-75 day-old control and genetically modified male mice after 48 hours in culture. RNA was amplified using the Affymetrix GeneChIP two-cycle protocol and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].

2010-03-01 | E-MEXP-1729 | biostudies-arrayexpress

Transcription profiling of liver from Hnf1a-deficient and wild type mice

Project description:RNA was extracted from Hnf1a/Tcf1 (Hepatocyte Nuclear Factor 1 alpha) knockout and wild-type mouse liver fractions. Gene expression profiles were compared using Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].

2009-03-21 | E-MEXP-1709 | biostudies-arrayexpress

Sex-dependent and HNF4alpha-dependent Mouse Liver Gene Expression

2008-03-21 | GSE10390 | GEO

Transcription profiling of mouse adult male wild-type liver (M-WT), adult female wild-type liver (F-WT), adult male liver-specific HNF4alpha knockout liver (M-KO) and adult female liver-specific HNF4alpha knockout liver (F-KO) to investigate sex-dependent and HNF4alpha-dependent gene expression

Project description:A series of dual-channel gene expression profiles obtained using Rosetta/Agilent Whole Mouse Genome oligonucleotide microarrays, 4 x 44K format, was used to identify sex-dependent and HNF4alpha-dependent differences in gene expression in adult mouse liver. This series is comprised of four sex-genotype combinations: adult male wild-type liver (M-WT), adult female wild-type liver (F-WT), adult male liver-specific HNF4alpha knockout liver (M-KO) and adult female liver-specific HNF4alpha knockout liver (F-KO). Four pools, each comprised of 4 randomly selected individual liver RNAs, were prepared for each sex-genotype combination. The pools were paired randomly to generate 4 separate experimental comparisons: M-WT:F-WT (first array comparison), M-WT:M-KO (second array comparison), F-WT:F-KO (third array comparison), and M-KO:F-KO (fourth array comparison). A total of 4994 HNF4alpha-dependent genes were identified, of which ~1000 fewer genes responded to the loss of HNF4alpha in female liver as compared to male liver. Moreover, 90% of the genes showing sex-specific expression in the liver were shown to lose sex specificity in HNF4alpha-deficient liver. Experiment Overall Design: An Alexa555-labeled cDNA sample is co-hybridized with an Alexa647-labeled cDNA sample. The samples are then dye-swapped and compared again on a second microarray chip. Together, these two mixed cDNA samples are considered a fluorescent reverse pair (dye swap). Similarly, a second fluorescent reverse pair is generated and the two pairs are averaged. The normalized expression ratio for each array is reported along with the two separate intensities. In this way, dye swaps were carried out for each of the four experimental comparisons. Thus, four microarrays, one for each mixed cDNA sample, were hybridized for each of the four fluorescent reverse pairs, giving a total of 16 microarrays.

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Comparison of Hepatocyte nuclear factor 4 alpha developmental and temporal knockout mice

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Total cellular proteome analysis of mutant and WT HNF4alpha Hepatic Progenitor Stage Cells

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2019-06-17 | PXD013737 | Pride

Comparison of Hepatocyte nuclear factor 4 alpha developmental and temporal knockout mice

2012-02-21 | E-GEOD-34581 | biostudies-arrayexpress

Transcriptional profiling of PNPase KO liver hepatocytes

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2018-05-03 | GSE109210 | GEO

Hepatic transcriptome profiling of liver-specific Elovl6 knockout mice fed a high-sucrose diet

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