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Transcription profiling of liver from Hnf1a-deficient and wild type mice

ABSTRACT: RNA was extracted from Hnf1a/Tcf1 (Hepatocyte Nuclear Factor 1 alpha) knockout and wild-type mouse liver fractions. Gene expression profiles were compared using Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].

ORGANISM(S): Mus musculus

DISEASE(S): mild hyperglycemia

SUBMITTER: Joan-Marc Servitja

PROVIDER: E-MEXP-1709 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hnf1alpha (MODY3) controls tissue-specific transcriptional programs and exerts opposed effects on cell growth in pancreatic islets and liver.

Servitja Joan-Marc JM Pignatelli Miguel M Maestro Miguel Angel MA Cardalda Carina C Boj Sylvia F SF Lozano Juanjo J Blanco Enrique E Lafuente Amàlia A McCarthy Mark I MI Sumoy Lauro L Guigó Roderic R Ferrer Jorge J

Molecular and cellular biology 20090316 11

Heterozygous HNF1A mutations cause pancreatic-islet beta-cell dysfunction and monogenic diabetes (MODY3). Hnf1alpha is known to regulate numerous hepatic genes, yet knowledge of its function in pancreatic islets is more limited. We now show that Hnf1a deficiency in mice leads to highly tissue-specific changes in the expression of genes involved in key functions of both islets and liver. To gain insights into the mechanisms of tissue-specific Hnf1alpha regulation, we integrated expression studies ...[more]

PMID: 19289501

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Transcription profiling of pancreatic islets from heterozygous Hnf1a-mutant and wild type mice

Project description:Pancreatic islets were isolated from Hnf1a+/- (Hepatocyte nuclear factor 1 alpha) and wild-type mice and cultured ex vivo for two days in RPMI medium with 10% FBS before RNA extraction. The transcription profiles were obtained using Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].

2009-03-21 | E-MEXP-1979 | biostudies-arrayexpress

Transcription profiling of pancreatic islets from Hnf1a-deficient and wild type mice

Project description:Pancreatic islets were isolated from Hnf1a (Hepatocyte nuclear factor 1 alpha) knockout and wild-type mice and cultured ex vivo for two days in RPMI medium with 10% FBS before RNA extraction. The transcription profiles were obtained using Affymetrix GeneChip Mouse Genome 430A 2.0 arrays [Mouse430A_2].

2009-03-21 | E-MEXP-1707 | biostudies-arrayexpress

Transcription profiling of pancreatic islets from wild type, pancreas-specific Hnf4alpha knockout, heterozygous Hnf1alpha, and double mutant mice

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2010-03-01 | E-MEXP-1729 | biostudies-arrayexpress

Transcription profiling of liver-specific Hnf4alpha knockout and wild type mouse liver

Project description:Gene expression differences between wild-type and liver-specific Hnf4alpha knockout (Albumin Cre+/-; Hnf4 fl/fl) mouse liver were assessed using RNA obtained from 2-month-old control and genetically modified female mice. RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].

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Human Pancreatic Islets Expressing HNF1A Variant Have Defective β cell Transcriptional Regulatory Networks

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2018-09-22 | GSE120299 | GEO

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Project description:Changes in gene expression in P. x canescens and P. euphratica in response to salt stress

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HNF1A, UTX, H3K27me3 and H3K27ac ChIP-seq in wild-type and UTX- or HNF1A-deficient mouse pancreas

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2019-12-20 | E-MTAB-7944 | biostudies-arrayexpress

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