Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling time series of lymph node, liver and spleen from two cattle breeds infected with Trypanosoma congolense to investigate breed differences in susceptibility


ABSTRACT: In this experiment, cattle from 2 breeds Boran and N'Dama were infected with Trypanosoma Congolense, IL1180 clone, African sleeping sickness, a disease which affects cattle in sub-saharan Africa. These breeds were chosen because they differ in their tolerance to infection, with Boran being highly susceptible, and N'Dama being more tolerant to infection. Three tissues Liver, Spleen and Lymph Node were harvested from individuals at various timepoints. Liver, Spleen and Lymph Node were collected post mortem at days 0 (naive), 21 and 35 post infection. In addition, further liver samples were collected by biopsy at days 0 (naive), 12, 15, 18, 26, 29, 32 days post infection. Each condition thus comprises: Time, Tissue, Breed. For each condition, RNA extracts from individuals were hybridised on individual arrays.
Infection experiment, tissue harvesting and RNA extraction were carried out at ILRI Nairobi Kenya.
Hybridizations were carried out at the Microarray facility, Roslin Institute, UK.
Data analysis was carried out at Liverpool University and Manchester University, UK.

ORGANISM(S): Bos taurus

SUBMITTER: Helen Hulme 

PROVIDER: E-MEXP-1778 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genetic and expression analysis of cattle identifies candidate genes in pathways responding to Trypanosoma congolense infection.

Noyes Harry H   Brass Andy A   Obara Isaiah I   Anderson Susan S   Archibald Alan L AL   Bradley Dan G DG   Fisher Paul P   Freeman Abigail A   Gibson John J   Gicheru Michael M   Hall Laurence L   Hanotte Olivier O   Hulme Helen H   McKeever Declan D   Murray Caitriona C   Oh Sung Jung SJ   Tate Catriona C   Smith Ken K   Tapio Miika M   Wambugu John J   Williams Diana J DJ   Agaba Morris M   Kemp Stephen J SJ  

Proceedings of the National Academy of Sciences of the United States of America 20110518 22


African bovine trypanosomiasis caused by Trypanosoma sp., is a major constraint on cattle productivity in sub-Saharan Africa. Some African Bos taurus breeds are highly tolerant of infection, but the potentially more productive Bos indicus zebu breeds are much more susceptible. Zebu cattle are well adapted for plowing and haulage, and increasing their tolerance of trypanosomiasis could have a major impact on crop cultivation as well as dairy and beef production. We used three strategies to obtain  ...[more]

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