Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of fission yeast mutants sep10 and sep15


ABSTRACT: The fission yeast Mcs6-Mcs2-Pmh1 complex (homologous to metazoan Cdk7-cyclin HMat1) performs dual functions: in cell cycle control, as a CDK-activating kinase; and in transcription by RNA polymerase (Pol) II, as part of TFIIH. It is unknown whether that linkage serves to coordinate gene expression with cell division. Mutants in mcs6 and pmh1 arrest with incomplete cell separation and decreased phosphorylation of the Pol II large subunit. Gene expression profiling by microarray hybridization revealed that a defined subset (~5%) of genes was repressed by Mcs6 complex impairment, whereas the majority was refractory. The repression signature included a cell-cycle cluster implicated in cytokinesis and cell separation, and overlapped with those of the cell-separation mutants sep1, sep10 and sep15 (relative to wild-type cells). The gene sep10 encodes the homolog of a protein found in metazoan Mediator-like complexes, and sep15 encodes an established component of the Mediator. In mcs6 or pmh1 mutants, sep10+, which also encodes a component of the transcriptional machinery, becomes essential for viability. Finally, mcs6+ also interacts genetically with sep1+, which encodes a forkhead transcription factor required for periodic transcription during mitosis and cell division. Thus, the Mcs6 complex (a direct activator of the cell-cycle engine) also helps govern the cell-cycle transcriptional program.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Karen Lee 

PROVIDER: E-MEXP-193 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Impairment of the TFIIH-associated CDK-activating kinase selectively affects cell cycle-regulated gene expression in fission yeast.

Lee Karen M KM   Miklos Ida I   Du Hongyan H   Watt Stephen S   Szilagyi Zsolt Z   Saiz Julia E JE   Madabhushi Ram R   Penkett Christopher J CJ   Sipiczki Matthias M   Bähler Jürg J   Fisher Robert P RP  

Molecular biology of the cell 20050413 6


The fission yeast Mcs6-Mcs2-Pmh1 complex, homologous to metazoan Cdk7-cyclin H-Mat1, has dual functions in cell division and transcription: as a partially redundant cyclin-dependent kinase (CDK)-activating kinase (CAK) that phosphorylates the major cell cycle CDK, Cdc2, on Thr-167; and as the RNA polymerase (Pol) II carboxyl-terminal domain (CTD) kinase associated with transcription factor (TF) IIH. We analyzed conditional mutants of mcs6 and pmh1, which activate Cdc2 normally but cannot complet  ...[more]

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