Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

48 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Listeria monocytogenes after infection into murine macrophages

ABSTRACT: P388D1 murine macrophages were cultured in 85 mm tissue culture plates to about semi confluency. L. monocytogenes serotypes (1/2a EGD-e, 4a L99, 4b CLIP80459 and 4b F2365) were infected to the P388D1 cell monolayer at a MOI of 100 per eukaryotic cell. Infection was carried for 45 min and followed by addition of fresh medium containing 20 M-5g/ml gentamicin. The medium of the plates (containing 20 M-5g/ml gentamicin) infected with L. monocytogenes serotypes were replaced after 2 h post infection with fresh medium containing 50 M-5g/ml gentamicin. At each step the plates were washed extensively with 1x PBS. Incubation of the bacterial tissue culture plates was carried out in a humidified incubator for up to 4 h post infection.

ORGANISM(S): Listeria monocytogenes

SUBMITTER: Torsten Hain

PROVIDER: E-MEXP-1947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of mouse cerebella from pups exposed to PTU to induce hypothyroidism

Project description:Pregnant dams were treated with 0.1% or 0.04% 6-propyl-2-thiouracil (PTU) in drinking water continuously from day 13 post conception until weaning to produce hypothyroid pups. Cerebella were collected from vehicle and 0.1% PTU treated pups at post-natal day (PND) 15 and mRNA from these was subjected to microarray analysis using Agilent high-density oligonucleotide chips.

2008-05-02 | E-MEXP-1048 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis wild type and choride channel d-1 mutant seedlings grown on various nitrogen sources

Project description:wild-type and mutant seedlings were grown on various Nitrogen sources

2009-03-01 | E-MEXP-1770 | biostudies-arrayexpress

CAGE_MPI_ahk_mutants

Project description:In-vitro-cultured seedlings of WT (Col-0) and all possible combinations of single, double, and triple T-DNA insertion mutants of the cytokinin receptors AHK2 (ahk2-5), AHK3 (ahk3-7) and AHK4 (cre1-2) at growth stage 1.00 were treated for 0, 15 and 120 min with 5micromolar of the synthetic cytokinin 6-benzyladednie (BA).

2013-07-04 | E-MEXP-670 | biostudies-arrayexpress

Transcriptomics of drougth stress in L. japonicus under normal air conditions

Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard (University Aarhus, Denmark) and then self-propagated at the University of Seville. Seeds were scarified and surface-sterilized, germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture of vermiculite and sand as solid support. Five seedlings were planted in each pot and grown during 35 days in a growth chamber under 16/8 hours day/night, 20/18M-oM-?M-=C, with a photosynthetic photon flux density of 250 M-oM-?M-=mol/m2M-oM-?M-=s and a constant humidity of 70%. Plants were watered with Hornum nutrient solution. Drought was applied withholding irrigation for the reported period of time and sample plants or leaves were harvested for further analysis.

2012-10-06 | E-MEXP-3710 | biostudies-arrayexpress

Seasonal and spatial influences on gene expression in Antarctic krill.

Project description:Effect of season and winter lattitude on gene expression in Antarctic krill.

2012-11-01 | E-MEXP-3453 | biostudies-arrayexpress

Effects of deletion of genes encoding RNA-binding proteins on gene expression in S. pombe

Project description:To study the contribution of RNA-binding proteins (RBPs) to the regulation of RNA turnover, we used DNA microarrays to examine the transcriptome of S. pombe strains containing deletions in non-essential genes encoding RBPs

2014-11-06 | E-MTAB-2314 | biostudies-arrayexpress

Effects of upf2delta and upf3delta on gene expression

Project description:The Upf1, Upf2 and Upf3 proteins cooperate to implement nonsense-mediated decay. We used DNA microarrays for profiling RNA expression levels of upf2 and upf3 mutants

2013-06-03 | E-MTAB-1325 | biostudies-arrayexpress

Effects of upf1delta on RNA stability

Project description:The Upf1 protein is a major factor in nonsense-mediated decay. We used an in vivo labelling system (Cleary et al. 2005 Nat Biotechnol. 23, 232-7) to estimate the decay rates of upf1delta and wild type cells over-expressing the transcription factor Mei4.

2013-06-03 | E-MTAB-1324 | biostudies-arrayexpress

Effects of deletion of RNA-binding proteins on RNA stability

Project description:To investigate the contribution of RNA-binding proteins to the regulation of RNA decay we used an in vivo labelling system (Cleary et al. 2005 Nat Biotechnol. 23, 232-7) to estimate the decay rates of RBP mutants and wild type S. pombe cells

2014-11-06 | E-MTAB-2712 | biostudies-arrayexpress

Ptp107 versus wild type Rlv3841

Project description:Strain PtsP107 has a Tn5 insertion in PtsP and it was grown on glucose/ ammonia minimal medium and gene expression compared to the wild type Rhizobium leguminosarum biovar viciae strain Rlv3841 grown on the same medium.

2011-09-23 | E-MEXP-3381 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data