Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

40 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of deletion of RNA-binding proteins on RNA stability

ABSTRACT: To investigate the contribution of RNA-binding proteins to the regulation of RNA decay we used an in vivo labelling system (Cleary et al. 2005 Nat Biotechnol. 23, 232-7) to estimate the decay rates of RBP mutants and wild type S. pombe cells

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Juan Mata

PROVIDER: E-MTAB-2712 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Effects of upf2delta and upf3delta on gene expression

Project description:The Upf1, Upf2 and Upf3 proteins cooperate to implement nonsense-mediated decay. We used DNA microarrays for profiling RNA expression levels of upf2 and upf3 mutants

2013-06-03 | E-MTAB-1325 | biostudies-arrayexpress

Effects of deletion of genes encoding RNA-binding proteins on gene expression in S. pombe

Project description:To study the contribution of RNA-binding proteins (RBPs) to the regulation of RNA turnover, we used DNA microarrays to examine the transcriptome of S. pombe strains containing deletions in non-essential genes encoding RBPs

2014-11-06 | E-MTAB-2314 | biostudies-arrayexpress

Effects of upf1delta on RNA stability

Project description:The Upf1 protein is a major factor in nonsense-mediated decay. We used an in vivo labelling system (Cleary et al. 2005 Nat Biotechnol. 23, 232-7) to estimate the decay rates of upf1delta and wild type cells over-expressing the transcription factor Mei4.

2013-06-03 | E-MTAB-1324 | biostudies-arrayexpress

Effects of upf1delta on gene expression

Project description:The Upf1 RNA-binding protein is essential for nonsense-mediated decay (NMD). We compared wild type (ura4-D18 leu1-32 h-) and upfdelta (upf1delta::kanMX6 ura4-D18 leu1-32 h-) cells transcriptome under normal growth conditions

2013-07-17 | E-MTAB-931 | biostudies-arrayexpress

Transcription profiling of fission yeast timecourse pmh1 mutant

Project description:The fission yeast Mcs6-Mcs2-Pmh1 complex (homologous to metazoan Cdk7-cyclin HMat1) performs dual functions: in cell cycle control, as a CDK-activating kinase; and in transcription by RNA polymerase (Pol) II, as part of TFIIH. It is unknown whether that linkage serves to coordinate gene expression with cell division. Mutants in mcs6 and pmh1 arrest with incomplete cell separation and decreased phosphorylation of the Pol II large subunit. Gene expression profiling by microarray hybridization revealed that a defined subset (~5%) of genes was repressed by Mcs6 complex impairment, whereas the majority was refractory. The repression signature included a cell-cycle cluster implicated in cytokinesis and cell separation, and overlapped with those of the cell-separation mutants sep1, sep10 and sep15 (relative to wild-type cells). The gene sep10 encodes the homolog of a protein found in metazoan Mediator-like complexes, and sep15 encodes an established component of the Mediator. In mcs6 or pmh1 mutants, sep10+, which also encodes a component of the transcriptional machinery, becomes essential for viability. Finally, mcs6+ also interacts genetically with sep1+, which encodes a forkhead transcription factor required for periodic transcription during mitosis and cell division. Thus, the Mcs6 complex (a direct activator of the cell-cycle engine) also helps govern the cell-cycle transcriptional program.

2004-11-01 | E-MEXP-191 | biostudies-arrayexpress

Transcription profiling of fission yeast timecourse mcs6 mutant

2004-11-01 | E-MEXP-192 | biostudies-arrayexpress

Role of the Ccr4/Not complex in the regulation of heterochromatin in Schizosaccharomyces pombe (IV)

Project description:Expression profiling of mutants of genes encoding components of the Ccr4-Not complex and other related genes

2015-09-25 | E-MTAB-3059 | biostudies-arrayexpress

Transcription profiling of fission yeast mutants sep10 and sep15

2004-11-01 | E-MEXP-193 | biostudies-arrayexpress

Transcription profiling of fission yeast with deletion or overexpression of ste11 and changes in gene expression after nitrogen starvation of h plus, h minus, h90fus1 and h90ste11 cells to investigate control of gene expression during early sexual differentiation

Project description:Fission yeast cells belong to one of two specialized cell types, M or P. Specific environmental conditions trigger sexual differentiation, which leads to an internal program starting with pheromone signalling between M and P cells, followed by mating, meiosis and sporulation. The initial steps of this process are controlled by Ste11p, a master transcriptional regulator that activates the expression of cell type-specific genes (only expressed in either M or P cells) as well as genes expressed in both M and P cells. <br><br> Pheromone signalling is activated by Ste11p-dependent transcription, and in turn enhances some of this transcription in a positive feedback. To obtain a genome-wide view of Ste11p target genes, their cell-type specificity, and their dependence on pheromone, we used DNA microarrays along with different genetic and environmental manipulations of fission yeast cells.We directly compared the transcriptome of homothallic wild-type cells (h90 fus1) with that of ste11 delta mutants under conditions that induce sexual differentiation. To allow for indirect effects of the ste11 delta mutation, we took advantage of the fact that ectopic expression of ste11 can drive cells into sexual differentiation and therefore is expected to cause the expression of Ste11p targets. We thus defined Ste11p targets as those genes whose expression was significantly reduced in a ste11 delta mutant and significantly increased when ste11 is overexpressed in vegetative cells.<br> <br> This study looked at the effect of deletion or overexpression of ste11, and changes in gene expression after nitrogen starvation of h plus, h minus, h90fus1 and h90ste11 cells.

2006-09-22 | E-TABM-139 | biostudies-arrayexpress

Transcription profiling of fission yeast as telomeres shorten in trt1- strains, and to compare survivors with linear and circular chromosomes

Project description:Time course experiment to watch RNA expression profiles as telomeres shorten in trt1- strains, and to compare survivors with linear and circular chromosomes.

2005-03-01 | E-MEXP-201 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data