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Nurf301 isoform requirements

ABSTRACT: Comparison of wild-type and Nurf301 mutant third instar larvae. Nurf301 mutants that remove all Nurf301 isoforms (Null) or mutants that remove full-length Nurf301 isoforms (DeltaC) were used

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Paul Badenhorst

PROVIDER: E-MEXP-2011 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Alternative splicing of NURF301 generates distinct NURF chromatin remodeling complexes with altered modified histone binding specificities.

Kwon So Yeon SY Xiao Hua H Wu Carl C Badenhorst Paul P

PLoS genetics 20090724 7

Drosophila NURF is an ISWI-containing chromatin remodeling complex that catalyzes ATP-dependent nucleosome sliding. By sliding nucleosomes, NURF can alter chromatin structure and regulate transcription. NURF301/BPTF is the only NURF-specific subunit of NURF and is instrumental in recruiting the complex to target genes. Here we demonstrate that three NURF301 isoforms are expressed and that these encode functionally distinct NURF chromatin remodeling complexes. Full-length NURF301 contains a C-ter ...[more]

PMID: 19629165

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