Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Drosophila exposed to a levitation magnet for different lengths of time

ABSTRACT: Drosophila samples were exposed to the levitation magnet inside a 25mm diameter tubes with 3 ml of yeast-based Drosophila food in the bottom and a chamber of only 5 mm of height over the food. This small region is needed in order to guarantee that all the flies were located in the effective g area so a maximum of 35 to 40 imagos or pupa can be exposed to each condition per experiment. All experiments were carried out with a parallel 1g external control in a temperature regulated incubator outside the magnet. Three experiments of different duration were performed inside the magnet system to analyze the effect of strong magnetic fields and magnetic levitation during different stages of the Drosophila development

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Raul Herranz

PROVIDER: E-MEXP-2082 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Microgravity simulation by diamagnetic levitation: effects of a strong gradient magnetic field on the transcriptional profile of Drosophila melanogaster.

Herranz Raul R Larkin Oliver J OJ Dijkstra Camelia E CE Hill Richard J A RJ Anthony Paul P Davey Michael R MR Eaves Laurence L van Loon Jack J W A JJ Medina F Javier FJ Marco Roberto R

BMC genomics 20120201

<h4>Background</h4>Many biological systems respond to the presence or absence of gravity. Since experiments performed in space are expensive and can only be undertaken infrequently, Earth-based simulation techniques are used to investigate the biological response to weightlessness. A high gradient magnetic field can be used to levitate a biological organism so that its net weight is zero.<h4>Results</h4>We have used a superconducting magnet to assess the effect of diamagnetic levitation on the f ...[more]

PMID: 22296880

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Project description:Genome-wide transcriptional profiling shows that reducing gravity levels in the International Space Station (ISS) causes important alterations in Drosophila gene expression. However, simulation experiments on ground, without space constraints, show weaker effects than space environment. A global and integrative analysis using the M-bM-^@M-^\gene expression dynamics inspectorM-bM-^@M-^] (GEDI) self-organizing maps, reveals a subtle response of the transcriptome using different populations and microgravity and hypergravity simulation devices. These results suggest that, in addition to behavioural responses that can be detected also at the gene expression level, the transcriptome is finely tuned to normal gravity. The alteration of this constant parameter on Earth can have effects on gene expression that depends both on the environmental conditions and the ground based facility used to compensate the gravity vector. Alternative and commons effects of mechanical facilities, like the Random Positioning Machine and a centrifuge, and strong magnetic field ones, like a cryogenically cooled superconductive magnet, are discussed. We compare the effects over the gene expression profile of different gender/age Drosophila imagoes in 3-4 days-long experiments under altered gravity conditions into three GBF ("Ground Based Facilities" for micro/hyper- gravity simulation) using whole genome microarray platforms. Descriptions of different GBFs ("treatments"): LDC means "Large Diameter Centrifuge". Samples can be placed under three conditions: inside LDC (at certain g level), at the LDC rotational control and at external 1g control (outside the LDC). RPM means "Random Positioning Machine". Samples can be placed under two conditions: inside RPM (at nearly 0g, Microgravity level) and at external 1g control (outside the RPM). At the magnet, means INSIDE the Magnetic levitator (another GBF). Samples can be placed under four conditions: inside Magnet 0g* (at microgravity with magnetic field), inside Magnet at 1g* (internal control with magnetic field) or inside the magnet 2g* (at hypergravity with magnetic field) and at external 1g control (outside the magnet)

Dataset Information

Transcription profiling of Drosophila exposed to a levitation magnet for different lengths of time

Publications

Microgravity simulation by diamagnetic levitation: effects of a strong gradient magnetic field on the transcriptional profile of Drosophila melanogaster.

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