Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

KLF10 targets

ABSTRACT: Experiment comparing the liver transcriptome from wild type and KLF10 deficient mice

ORGANISM(S): Mus musculus

SUBMITTER: Franck Delaunay

PROVIDER: E-MEXP-2089 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Kruppel-like factor KLF10 is a link between the circadian clock and metabolism in liver.

Guillaumond Fabienne F Gréchez-Cassiau Aline A Subramaniam Malayannan M Brangolo Sophie S Peteri-Brünback Brigitta B Staels Bart B Fiévet Catherine C Spelsberg Thomas C TC Delaunay Franck F Teboul Michèle M

Molecular and cellular biology 20100412 12

The circadian timing system coordinates many aspects of mammalian physiology and behavior in synchrony with the external light/dark cycle. These rhythms are driven by endogenous molecular clocks present in most body cells. Many clock outputs are transcriptional regulators, suggesting that clock genes primarily control physiology through indirect pathways. Here, we show that Krüppel-like factor 10 (KLF10) displays a robust circadian expression pattern in wild-type mouse liver but not in clock-def ...[more]

PMID: 20385766

Similar Datasets

Transcription profiling of mouse pancreatic P03 adenocarcinoma to study the effect of meal timing.

Project description:B6D2F1 male mice at the age of 6 weeks were maintained for one week in a 12h light / 12 h dark (LD12:12) cycle (lights on from 7:00 am to 7:00 pm) and food and water ad libitum. Mice were then divided in two experimental groups which were further maintained for 3 weeks in the LD12 cycle and fed either at libitum or only during a 4 h period between 9:00 am and 1:00 pm. All animals were then implanted subcutaneously with a pancreatic P03 adenocarcinoma in both flanks. Tumour growth was monitored daily and twenty one days after innoculation, animals were transfered to constant darkness for 24h. Tumour samples were collected at the implantation site at circadian time (CT)4 and CT16.

2010-05-07 | E-MEXP-2462 | biostudies-arrayexpress

Transcription profiling of porcine alveolar macrophages in response to Streptococcus suis serotype 2

Project description:Incubation of porcine alveolar macrophages with two different strains of streptococcus suis and a control

2008-08-19 | E-MEXP-1756 | biostudies-arrayexpress

RNAi profiling of human MDA-MB-231 cells knocked down for RhoGDIbeta

Project description:RhoGDIbeta (ARHGDIB) is often expressed in tumor cells. It negatively regulates Rho-GTPases, but may have other functions as well. To analyze its effect on gene expression, RhoGDIbeta was suppressed by RNA interference in MDA-MB-231 breast cancer cells and changes in gene expression monitored by cDNA microarrays. Experiment Overall Design: MDA-MB-231 cells were either transfected with a RhoGDIbeta-specific siRNA or a control siRNA (siLuc), grown for three days, harvested and lysed for total RNA isolation. RNA was subjected to cDNA microarray analysis by using Affymetrix chip HG-U133A. A total of three independent transfection experiments were performed. The samples obtained from experiments 1, 2 and 3 were designated as siLuc_rep1, siLuc_rep2, siLuc_rep3 (siLuc transfected) and siARH_rep1, siARH_rep2, siARH_rep3 (siRhoGDIbeta transfected), respectively.

2008-06-15 | E-GEOD-8087 | biostudies-arrayexpress

Transcription profiling time series of tomato leaves after infection with a foliar and a vascular fungal pathogen

Project description:Time course of Cladosporium fulvum and Verticillium dahliae infected tomato.

2009-04-14 | E-MEXP-1844 | biostudies-arrayexpress

Genotyping of human glioma

Project description:Study of extrachromosomal amplification mechanisms in a glioma

2010-01-26 | E-MEXP-2423 | biostudies-arrayexpress

Transcription profiling by array of mouse M1 cells expressing Batf targeting siRNA or control siRNA and treated with leukemia inhibitory factor to identify putative downstream targets of Batf

Project description:In order to identify putative downstream targets of Batf in M1 cells, a microarray analysis was performed. Briefly, M1/Neg-RNAi (expressing control siRNA with no mammalian target) and M1/Batf-RNAi cells (expressing Batf targeting siRNA) were seeded in M1 growth medium at a density of 3 X 105/ml in 100mm culture dishes. Six plates were plated for each cell type. Within each group, three were labeled as + LIF and treated with leukemia inhibitory factor (LIF) (5 ng/ml) for 6 hours the following day. After 6 hours, the cells were harvested from all plates individually and RNA prepared. 5 µg of total RNA from the three individual samples representing each subgroup (M1/Neg-RNAi + LIF, - LIF; M1/Batf-RNAi + LIF, - LIF) were combined into one pool of RNA. EJT001 represents sample "M1/Neg-RNAi, no LIF)"; EJT002 represents sample "M1/Neg-RNAi, with LIF)"; EJT003 represents sample "M1/Batf-RNAi, no LIF)"; EJT004 represents sample "M1/Batf-RNAi, with LIF)". The four pools of RNA were shipped to Genome Explorations, Inc. for analysis using Affymetrix GeneChip technology. The differentially regulated genes were identified both within each group (+ LIF compared to - LIF) and between 2 goups (M1/Neg-RNAi and M1/Batf-RNAi).

2011-03-01 | E-MEXP-2995 | biostudies-arrayexpress

Transcription profiling of Arabidopsis XW119 seedlings 0-4 hours after ethanol induction

Project description:4-day-old XW119 seedlings were treated with 2% Ethanol on MS agar plates under light, and samples were collected at 0, 1, 2, 4 hours.

2010-04-19 | E-MEXP-2229 | biostudies-arrayexpress

Transcription profiling of wild_type and IGF-I receptor over-expressing mouse lung carcinoma cells identifies a type I insulin like growth factor receptor regulated gene expression profile associated with an altered site-specificity of metastasis.

Project description:Identification of a type I insulin like growth factor receptor regulated gene expression profile associated with an altered site-specificity of metastasis.

2008-11-01 | E-MEXP-1361 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type, MYB46 over-expressing, MYB83 over-expressing and myb46/myb83 double mutants to investigate the regulation mechanism of secondary cell wall synthesis during xylem vessel differentiation

Project description:To investigate the regulation mechanism of secondary cell wall synthesis during xylem vessel differentiation, we analyzed the function of two Arabidopsis MYB transcription factors, MYB46 and MYB83. Microarray analysis was performed for 11-day old seedlings of wild-type, MYB46 over-expressor, MYB83 over-expressor, and myb46 myb83 double mutant using Affymetrix ATH-1 Genome Array. Gene expressions in MYB46 over-expressor, MYB83 over-expressor, and myb46 myb83 double mutant were compared to those in wild-type.

2010-05-01 | E-MEXP-2445 | biostudies-arrayexpress

Transcription profiling of intestine from wild type and Mdm2 knock-out mice

Project description:Analyzed gene expression in 3-day old mice lacking Mdm2 in the intestine

2008-03-23 | E-MEXP-1431 | biostudies-arrayexpress