Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of wounds from ovariectomized MIF null mice and controls to investigate the role of MIF during wound healing using BALB/C MIF null mice

ABSTRACT: The aim of this experiment was to investigate the role of MIF during wound healing using BALB/C MIF null mice and in the context of reduced estrogen-associated impaired healing using ovariectomized mice (a mouse model of age-associated delayed healing). Ageing is associated with delayed cutaneous wound healing resulting from reduced estrogen levels. Macrophage migration inhibitory factor (MIF - NCBI RefSeq: NM_010798) is thought to mediate the effects of estrogen on wound healing. Gene expression was compared between wounds from ovariectomized MIF null mice and controls.

ORGANISM(S): Mus musculus

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-209 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Macrophage migration inhibitory factor: a central regulator of wound healing.

Hardman Matthew J MJ Waite Alexander A Zeef Leo L Burow Matthew M Nakayama Toshinori T Ashcroft Gillian S GS

The American journal of pathology 20051201 6

Age-associated differences in estrogen levels critically modify the cutaneous wound healing response. Using a microarray-based approach, we profiled changes in gene expression within the wounds of mice that were wild type or null for the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) in the presence or absence of estrogen. This experimental design identified more than 600 differentially expressed genes and established MIF as a key player in the wound healing process, regu ...[more]

PMID: 16314470

Similar Datasets

Transcription profiling of diabetic neuropathy in dorsal root ganglia from streptozotocin-diabetic male wistar rats over the first 8 weeks of diabetes

Project description:A study of diabetic neuropathy in dorsal root ganglia from streptozotocin-diabetic male wistar rats over the first 8 weeks of diabetes

2006-12-10 | E-MEXP-515 | biostudies-arrayexpress

Transcription profiling of mouse long-lived S6K1 -/- strain females compared to wild types

Project description:We examined the transcript changes in long-lived S6K1-/- female mice compared to wild type controls.

2009-09-04 | E-MEXP-2320 | biostudies-arrayexpress

Transcription profiling of mouse HoxA2 knockout

Project description:Transcription profiling was performed of second branchial arches of E11.5 embryos from Hoxa2+/- intercrosses. After genotyping the embryos, wild type and Hoxa2-/- were profiled by microarray.

2011-12-01 | E-MEXP-3261 | biostudies-arrayexpress

Experimental overactivity or blockade of transforming growth factor-B each generate a specific ureter malformation

Project description:The mammalian ureter is a tube connecting the kidney with the urinary bladder. The differentiated urothelial tube is surrounded by smooth muscle cells (SMCs). In mice embryos by E15, the first SM cells have formed and, over the next few prenatal days, the ureter becomes a functional unit, transmitting urine generated by the metanephros. Molecules in the transforming growth factor-beta (TGFB) axis have been reported to be upregulated in both kidney and ureter malformations. This study reports the effects of TGFB1 treatment on E15 mouse ureters in vitro.

2019-08-07 | E-MTAB-7395 | biostudies-arrayexpress

Altered dermal architecture in PKCα-/- skin

Project description:Global gene expression analysis in PKCα-/- mouse skin reveals structural changes in the dermis and defective wound granulation tissue. The skin's mechanical integrity is maintained by an organised and robust dermal extracellular matrix (ECM). Resistance to mechanical disruption hinges primarily on homeostasis of the dermal collagen fibril architecture which is regulated, at least in part, by members of the small leucine-rich proteoglycan (SLRP) family. This array study presents data linking protein kinase C alpha (PKCα) to the regulated expression of multiple ECM components including SLRPs.

2016-01-20 | E-MTAB-3836 | biostudies-arrayexpress

A novel epidermal-specific role for arginase1 during cutaneous wound repair

Project description:Non-healing wounds are a major area of unmet clinical need that remain problematic to treat; therefore, improved understanding of pro-healing mechanisms is invaluable. The enzyme arginase1 is involved in pro-healing responses with its role in macrophages best-characterised. Arginase1 is also expressed by keratinocytes; however, the function of arginase1 in these critical wound repair cells is not understood. We characterised arginase1 expression in keratinocytes during normal cutaneous repair and reveal de novo temporal and spatial expression at the epidermal wound edge. Interestingly, epidermal arginase1 expression was decreased in both human and murine delayed healing wounds. We, therefore, generated a keratinocyte specific arginase1-null mouse model (K14-cre;Arg1fl/fl) to explore arginase function. Wound repair, linked to changes in keratinocyte proliferation, migration and differentiation, was significantly delayed in K14-cre;Arg1fl/flmice. Gene expression was studied by microarray.

2021-07-07 | E-MTAB-10213 | biostudies-arrayexpress

Transcription profiling of rat model of nonischemic cerebral hypoperfusion to investigate molecular mechanisms of early response in adaptive cerebral arteriogenesis

Project description:This study aims at a comprehensive understanding of the genomic program activated during early-phase of collateral vessel growth in a rat model for cerebral adaptive arteriogenesis (3-VO). While arteriogenesis constitutes a promising therapeutic concept for cerebrovascular ischemia, genomic profiles essential for therapeutic target identification were analysed solely for collateral arteries of the heart and periphery. Despite challenging anatomical conditions of the brain the 3-VO model allows identification of differentially expressed genes during adaptive cerebral arteriogenesis by selective removal of the posterior cerebral artery (PCA). Experiment Overall Design: Using an established rat model of nonischemic cerebral hypoperfusion (3-VO) (Busch, Buschmann; 2003), RNA was extracted from isolated ipsilateral PCA. Pooled RNAs from groups of intact (0h), sham and 3-VO animals 24h and 3 days after surgery, were hybridised repeatedly for an extensive genome screen of 15866 genes applying standardized Affymetrix technology. For each Array total RNA from 8 animals was processed, pooled and hybridized to a Rat230A GeneChip per group. These groups were classified as follows: intact control (N=3), 24h3VO (N=3); 24h sham (N=3), 3days3VO (N=3); and 3days sham(N=3). Hybridization, washing, antibody amplification, staining, and scanning of probe arrays were performed according to the Affymetrix Technical Manual. Probe arrays were scanned using the GeneChip System (Hewlett-Packard, Santa Cruz, CA)(Affymetrix) and raw data were processed using GCOS and normalized to a global intensity of 500.

2008-10-25 | E-GEOD-6189 | biostudies-arrayexpress

Mouse F2 Cross, Carbohydrate Diet, Fat Tissue

Project description:Gene transcription in epididymal fat pads was investigated in an F2 cross of 129S6 x Balb/c mice using Illumina gene expression arrays. Expression data was determined in 5 months old male mice fed a carbohydrate diet. Genotyping data for the F2 offspring used in this study can be found in the file E-MTAB-395.additional.zip under the Browse all available files link (file mouse-f2-chd-genotype.txt added 8th April 2014).

2014-01-20 | E-MTAB-395 | biostudies-arrayexpress

Microarray examination of liver tissue from mice fed 58% saturated high fat diet to induce hepatic steatosis.

Project description:The molecular biology behind hepatic steatosis is unknown. This analysis was done as part of an experiment to understand the role changes in the epigenome have on disease progression. It was done in conjunction with DNA immunoprecipitation sequencing to look at how DNA cytosine species are changed in the context of induction of gene expression.

2017-05-31 | E-MTAB-5520 | biostudies-arrayexpress

Transcription profiling of mouse kidney following infection with virulent or attenuated strains of Candida albicans

Project description:Comparison of transcriptional responses of the mouse kidney to infections by virulent or attenuated strains of Candida albicans

2009-10-01 | E-MEXP-1458 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data