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Transcription profiling of human benign and malignant pheochromocytomas

ABSTRACT: Total RNA was isolated from fresh-frozen tissue of 5 benign and 5 malignant pheochromocytomas. The reference consisted of laser microdissected (LMD) tissue from normal adrenal medullae from 10 different patients. After generating Cy3- and Cy5-fluorescently labeled cDNAs, F-chips containing 11,540 spots were hybridized. Data were analyzed with the IMAGENE 3.0 software.

ORGANISM(S): Homo sapiens

DISEASE(S): myelolipoma

SUBMITTER: Jens Waldmann

PROVIDER: E-MEXP-2137 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray analysis reveals differential expression of benign and malignant pheochromocytoma.

Waldmann Jens J Fendrich Volker V Holler Julia J Buchholz Malte M Heinmöller Ernst E Langer Peter P Ramaswamy Annette A Samans Birgit B Walz Martin K MK Rothmund Matthias M Bartsch Detlef K DK Slater Emily P EP

Endocrine-related cancer 20100728 3

The diagnosis of a malignant pheochromocytoma (PC) can only be established by the presence of distant metastases, but a subset of apparently benign PCs develop metastases. We have employed a microarray analysis to identify a typical gene expression profile which distinguishes malignant from benign PC. Total RNA was isolated from fresh-frozen tissue of five benign and five malignant PCs. The reference consisted of laser microdissected tissue from normal adrenal medulla. After generating Cy3- and ...[more]

PMID: 20562231

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