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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human BJ fibroblasts after LIN-9 depletion by a shRNA

ABSTRACT: Aim: identification of differentially expressed genes after LIN-9 depletion.

hTERT immortalized human BJ fibroblasts were infected with pMSCV-Blasticidin based retroviruses encoding a shRNA which targets human LIN-9 and the empty vector respectively.
After 4 days of selection total RNA was isolated. Cy3 and Cy5 labelled cDNA probes were generated using the CyScribe Post-labelling Kit (Amersham Biosiences).

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens

SUBMITTER: Stefanie Hauser

PROVIDER: E-MEXP-752 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The human synMuv-like protein LIN-9 is required for transcription of G2/M genes and for entry into mitosis.

Osterloh Lisa L von Eyss Björn B Schmit Fabienne F Rein Lena L Hübner Denise D Samans Birgit B Hauser Stefanie S Gaubatz Stefan S

The EMBO journal 20061207 1

Regulated gene expression is critical for the proper timing of cell cycle transitions. Here we report that human LIN-9 has an important function in transcriptional regulation of G2/M genes. Depletion of LIN-9 by RNAi in human fibroblasts strongly impairs proliferation and delays progression from G2 to M. We identify a cluster of G2/M genes as direct targets of LIN-9. Activation of these genes is linked to an association between LIN-9 and B-MYB. Chromatin immunoprecipitation assays revealed bindi ...[more]

PMID: 17159899

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