Ontology highlight
ABSTRACT:
hTERT immortalized human BJ fibroblasts were infected with pMSCV-Blasticidin based retroviruses encoding a shRNA which targets human LIN-9 and the empty vector respectively.
After 4 days of selection total RNA was isolated. Cy3 and Cy5 labelled cDNA probes were generated using the CyScribe Post-labelling Kit (Amersham Biosiences).
INSTRUMENT(S): 418 [Affymetrix]
ORGANISM(S): Homo sapiens
SUBMITTER: Stefanie Hauser
PROVIDER: E-MEXP-752 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
The EMBO journal 20061207 1
Regulated gene expression is critical for the proper timing of cell cycle transitions. Here we report that human LIN-9 has an important function in transcriptional regulation of G2/M genes. Depletion of LIN-9 by RNAi in human fibroblasts strongly impairs proliferation and delays progression from G2 to M. We identify a cluster of G2/M genes as direct targets of LIN-9. Activation of these genes is linked to an association between LIN-9 and B-MYB. Chromatin immunoprecipitation assays revealed bindi ...[more]