Project description:Changes in gene expression in P. trichocarpa leaves was measured in response to growth competition by P. x canescens.Square petri dishes (12 x 12 cm) were filled autoclaved sand, 3 plantlets and Woody Plant Medium (WPM). In each petri dish we are growing 3 plantlets. As control 3 plantlets of P. trichocarpa and for the experiment a mixture of P. canescens and P. trichocarpa were P. trichocarpa is all the time the middle plantlet.
Project description:Changes in gene expression in P. trichocarpa leaves was measured in response to growth competition by P. x canescens.Square petri dishes (12 x 12 cm) were filled autoclaved sand, 3 plantlets and Woody Plant Medium (WPM). In each petri dish we are growing 3 plantlets. As control 3 plantlets of P. trichocarpa and for the experiment a mixture of P. canescens and P. trichocarpa were P. trichocarpa is all the time the middle plantlet.
Project description:To better understand the spatial distribution of gene expression network in legume roots, transcriptomics profiles of border cells, root tips and whole roots were compared.
Project description:Populus x canescens was inoculated with Paxillus involutus and grown in a climate chamber for 13 weeks. Afterwards, plants were salt stressed for 18 additional days with 150 mM NaCl in the nutrient solution. This resulted in 4 different treatments: no mycorrhiza<br>control (NC), mycorrhiza/control (MC), no mycorrhiza/salt (NS), mycorrhiza/salt (MS).
Project description:Arabidopsis seedlings, of both wild-type and an ARF7/ARF19 double knockout mutant, were grown to 7 days post-germination. The roots were then dissected into 5 developmental zones, the meristem, early elongation zone, late elongation zone, mature root and lateral root zone. The sections then underwent transcriptional profiling to identify processes and regulatory events specific and in common to the zones.
Project description:This experiment was designed to study the interactions between Medicago truncatula and the charcoal rot pathogen Macrophomina phaeolina. Two-week-old plants grown in Magenta boxes supplied with 1/2 MS salt and 1% sucrose were inoculated with M. phaseolina covered wheat seeds, and roots were harvested at 24, 36 and 48 hours after inoculation. Control plants were mock inoculated with a sterile wheat seed, and roots were harvest 24 hours later. Pooled RNAs were used in the array experiment using Affymetrix GeneChip(r) Medicago Genome Array.
Project description:A BAP (benzylaminopurine, cytokinin) solution (10-7 M) was applied to root tips of Medicago truncatula (Jemalong A17 genotype) for 1h. Microarrays were used to compare the transcriptome of these plants (4 replicates) with the transcriptome of control plants, not exposed to BAP (4 replicates)
Project description:A Salt stress (100mM) was applied to root tips of Medicago truncatula (Jemalong A17 genotype) for 1h. Microarrays were used to compare the transcriptome of these plants (4 replicates) with the transcriptome of control plants, not submitted to the salt stress (4 replicates)