Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse embryonic stem cell line CGR8 transfected with control shRNA and shRNA against HP1g in two different developmental stages

ABSTRACT: The work was done one mouse embryonic stem cells.
Three independent experiments were carried out.
Two cell lines were used. They derive from the same cell line (CGR8). ShCTR is a cell line transduced with a control shRNA. ShN2 is a cell line transduced with a shRNA against HP1g.
The different stage of these cell lines were analysed : proliferating undifferentiated cells (ES) and differentiated cells (EB36h).

ORGANISM(S): Mus musculus

SUBMITTER: Maia Caillier

PROVIDER: E-MEXP-2238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.

Caillier Maïa M Thénot Sandrine S Tribollet Violaine V Birot Anne-Marie AM Samarut Jacques J Mey Anne A

PloS one 20101115 11

The unique properties of embryonic stem cells (ESC) rely on long-lasting self-renewal and their ability to switch in all adult cell type programs. Recent advances have shown that regulations at the chromatin level sustain both ESC properties along with transcription factors. We have focused our interest on the epigenetic modulator HP1γ (Heterochromatin Protein 1, isoform γ) that binds histones H3 methylated at lysine 9 (meH3K9) and is highly plastic in its distribution and association with the t ...[more]

PMID: 21085495

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Project description:Phenotypic plasticity, the ability of one genotype to express different phenotypes in response to changing environmental conditions, is one of the most common phenomena characterising the living world and is not only relevant for the ecology but also for the evolution of species. Daphnia, the waterflea, is a textbook example for predator induced phenotypic plastic defences including changes in life-history, behaviour and morphology. However, the analysis of molecular mechanisms underlying these inducible defences is still in its early stages. We exposed Daphnia magna to chemical cues of the predator Triops cancriformis to identify key processes underlying plastic defensive trait formation. D. magna is known to develop an array of morphological changes in the presence of T. cancriformis including changes of carapace morphology and cuticle hardening. To get a more comprehensive idea of this phenomenon, we studied four different genotypes originating from habitats with different predation history, reaching from predator-free to temporary habitats containing T. cancriformis. We analysed the morphologies as well as proteomes of predator-exposed and control animals. Three genotypes showed morphological changes when the predator was present. Using a high-throughput proteomics approach, we found 294 proteins which were significantly altered in their abundance after predator exposure in a general or genotype dependant manner. Proteins connected to genotype dependant responses were related to the cuticle, protein synthesis and calcium binding whereas the yolk protein vitellogenin increased in abundance in all genotypes, indicating their involvement in a more general response. Furthermore, genotype dependant responses at the proteome level correlated well with local adaptation to Triops predation. Altogether, our study provides new insights concerning genotype dependant and general molecular processes involved in predator-induced phenotypic plasticity in D. magna.

Dataset Information

Transcription profiling of mouse embryonic stem cell line CGR8 transfected with control shRNA and shRNA against HP1g in two different developmental stages

Publications

Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.

Similar Datasets

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