Chromatin immunoprecipitation to identify Ino80 binding sites in yeast following phosphate startvation

ABSTRACT: ChIP on CHIP analysis were performed to analyse the direct involvement of Ino80 in the regulation of the phosphate responsive and nucleotide metabolism genes upon phosphate starvation: We immunoprecipitated Ino80-bound chromatin fragments after formaldehyde crosslinking from cells grown in minimal medium (EMM) or minimal medium lacking phosphate and purified, amplified and probed the Ino80-associated DNA using oligonucleotide tiling arrays.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Mickael Durand-Dubief 

PROVIDER: E-MEXP-2284 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress