Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of aortic samples from newborn and 6 week old C57/black6 wild type mice

ABSTRACT: Aortic samples ranging from the aortic valve to the diaphragm were harvested from 15 newborn and 15 six-week old C57/black6 wildtype mice. Frozen samples of 5 animals each were pulverized and directly dissolved in RNA pure (PeqLab) to get three samples for each group. Total RNA was isolated using phenol/chloroform extraction.

For gene-expression analysis, 500 ng total RNA of each RNA sample was labeled using the Agilent single-color Quick-Amp Labeling Kit and hybridized on Agilent Whole Mouse Genome Microarrays (4x44K). For

analysis of microRNA, 200 ng total RNA of each sample was labeled using the Agilent miRNA Labeling Reagent and Hyb Kit, and hybridized on Agilent Mouse miRNA Microarrays (8x15K) according to the manufacturer's instructions.

ORGANISM(S): Mus musculus

SUBMITTER: Marten Jaeger

PROVIDER: E-MEXP-2342 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

The purpose of the experiment was to study miRNA expression in prostate cancer cell lines and xenografts and to combine it with miRNA gene copy number data that we already had to identify miRNAs that could be overexpressed or underexpressed as a consequence of amplification or deletion of the miRNA gene, respectively.

Project description:miRNA expression profiling in prostate cancer cell lines and xenografts

2011-06-30 | E-MEXP-2966 | biostudies-arrayexpress

Identification of mirna during osteoblast differentiation of human dental pulp stem cells

Project description:To evaluate the miRNA and mRNA expression profiles (miRNOME) we identified miRNAs during in vitro osteogenic differentiation of human dental pulp stem cells (DPSC). The DPSCs were cultured in the DMEM + beta-glycerol phosphate, ascorbic acid and dexamethasone for 2 dias to 21days. The microRNA or mRNA expression profiling during the differentiation process was analyzed through hybridizations with Agilent miRNA-microarray (8x15K format).

2014-11-28 | E-MTAB-3077 | biostudies-arrayexpress

miRNA profiling of mixoid liposarcoma cell lines sensitive and resistant to trabectidin

Project description:This investigation aims at identifying differentially expressed miRNA between two cell lines, MLS 402-91 (sensitive to trabectidin) and MLS 402-91/ET (resistant to trabectidin).

2012-06-05 | E-MTAB-695 | biostudies-arrayexpress

Transcriptional profiling by array of microRNAs from human peripheral blood mononuclear cells - PBMC - of Type 2 diabetes mellitus patients

Project description:The microRNA oligo microarrays were used to determine expression profiles of peripheral blood mononuclear cells from type 2 diabetes mellitus (T2DM) patients, aiming the identification of possible disease related MicroRNAs.

2015-04-01 | E-MTAB-2899 | biostudies-arrayexpress

Transcriptional profiling by array of microRNAs of human peripheral blood mononuclear cells - PBMC - from control individuals for Type 2 diabetes mellitus patients

Project description:The microRNA oligo microarrays were used to determine expression profiles of peripheral blood mononuclear cells from control healthy individuals and compare to type 2 diabetes mellitus (T2DM) patients, aiming the identification of possible disease related MicroRNAs.

2015-04-01 | E-MTAB-2902 | biostudies-arrayexpress

Transcriptional profiling by microarray of microRNAs of osteoblastic cells from calvaria and bone marrow of ovariectomized rats.

Project description:The microRNA oligo microarrays from Agilent were used to evaluate behavioral differences in osteoblast-like cells from calvaria and bone marrow of rats induced to osteoporosis by ovariectomy. The miRNAs data were normalized using the quantile and after statistical analysis by moderate t-test with p-value ≤ 0.05 and subsequent fold change ≥ 1.5, 84 miRNAs differentially expressed in calvaria cells were detected and 55 miRNAs in bone marrow cells.

2015-05-25 | E-MTAB-3563 | biostudies-arrayexpress

Transcriptional profiling by microarray of mRNAs of osteoblastic cells from calvaria and bone marrow of ovariectomized rats.

Project description:The mRNA oligo microarrays from Agilent were used to evaluate behavioral differences in osteoblast-like cells from calvaria and bone marrow of rats induced to osteoporosis by ovariectomy. Microarray data were normalized using quantile and after statistical analysis by unpaired t-test with p-value ≤ 0.005 and subsequent fold change ≥ 2.0, 5.447 differentially expressed mRNAs were detected in calvaria cells and 4.399 mRNAs in bone marrow cells.

2015-05-25 | E-MTAB-3564 | biostudies-arrayexpress

The effect of the Autoimmune regulator (Aire) gene on microRNA transcriptional profile of 3.10 mTEC cell line

Project description:Medullary thymic epithelial cells (mTECs) contribute to self-tolerance through the ectopic expression of peripheral tissue antigens (PTAs) in the thymus. Autoimmune regulator (Aire) is to date the best characterized transcriptional regulator known to at least partially coordinate PTA expression in mTECs. Furthermore the expression of Aire-dependent and -independent genes is also modulated by microRNAs. Given the transcriptional regulation exerted by Aire in mTEC cells and the role of miRNAs in post-transcriptional control, we used a Mus musculus mTEC cell line (3.10) which constitutively express Aire in culture) to knockdown Aire gene by means of siRNA transfection and then observe the effect of Aire knockdown in the transcriptional profile of miRNAs by means of oligomicroarrays. The Agilent oligomicroarrays were used to determine the large scale the miRNA transcriptional profiles of control or Aire-knockdown 3.10 mTECs.

2016-06-27 | E-MTAB-4596 | biostudies-arrayexpress

The effect of Dicer knockdown on the microRNA transcriptional profile of 3.10 mTEC cell line.

Project description:Dicer, also known as endoribonuclease Dicer or helicase with RNase motif, cleaves double-stranded RNA (dsRNA) and pre-microRNA (pre-miRNA) into short mature double-stranded RNA fragments called small interfering RNA and microRNA, respectively. MicroRNAs are potent post-transcriptional regulators in developmental switches, lineage commitment and gene expression regulation. To evaluate their post-transcriptional role in gene expression of Medullary thymic epithelial cells (mTECS) we knocked down Dicer transcript by means of siRNA transfection in a Mus musculus mTEC cell line (3.10 mTEC). The Agilent oligo microarrays were used to determine the large scale microRNA (miRNA) transcriptional profiles of control or Dicer-knockdown 3.10 mTECs.

2016-06-27 | E-MTAB-4627 | biostudies-arrayexpress

Transcriptional profiling by microarray of microRNAs of pancreas-infiltrating T lymphocytes from NOD mice

Project description:The microRNA oligo microarrays from Agilent were used to determine miRNA expression profile of pancreas-infiltrating T CD3+ and total T lymphocytes of NOD mice.

2015-05-29 | E-MTAB-3556 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data