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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of aortic samples from newborn and 6 week old C57/black6 wild type mice

ABSTRACT: Aortic samples ranging from the aortic valve to the diaphragm were harvested from 15 newborn and 15 six-week old C57/black6 wildtype mice. Frozen samples of 5 animals each were pulverized and directly dissolved in RNA pure (PeqLab) to get three samples for each group. Total RNA was isolated using phenol/chloroform extraction.

For gene-expression analysis, 500 ng total RNA of each RNA sample was labeled using the Agilent single-color Quick-Amp Labeling Kit and hybridized on Agilent Whole Mouse Genome Microarrays (4x44K). For

analysis of microRNA, 200 ng total RNA of each sample was labeled using the Agilent miRNA Labeling Reagent and Hyb Kit, and hybridized on Agilent Mouse miRNA Microarrays (8x15K) according to the manufacturer's instructions.

ORGANISM(S): Mus musculus

SUBMITTER: Marten Jaeger

PROVIDER: E-MEXP-2342 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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