Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

44 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of human SKBR-3 cells VE-cadherin siRNA and then treated with retinoic acid

ABSTRACT: SKBR-3 cells were transfected with Luciferase siRNA or VE-cadherin siRNA and then treated with 10-7M retinoic acid (RA) for 48 hours.

ORGANISM(S): Homo sapiens

SUBMITTER: Priya Prahalad

PROVIDER: E-MEXP-2418 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of homo sapiens (human) SKBR-3 cells treated with 0.1 uM RA vs controls

Project description:SKBR-3 cells were treated with control or 0.1uM RA for 48 hours

2009-11-30 | E-MEXP-2417 | biostudies-arrayexpress

Human breast cancer SKBr-3 cells: trastuzumab-resistant vs. parental

Project description:Human breast cancer SKBr-3 cells selected with trastuzumab for 6 month compared with parental SKBr-3 cells; Goal was to screen for microRNAs involved in development of trastuzumab resistance. Human breast cancer SKBr-3 cells were continuously cultured in the presence ofM-BM- 5M-BM-5g/ml trastuzumab for 6 months, and the resulting alive cells were regarded as trastuzumab-resistant. Total RNAs were prepared from these cells and cells cultured in parallel in control media, and were subjected to hybridization on the miRCURY LNA Array (Exiqon, version 11.0).

2013-05-25 | E-GEOD-47011 | biostudies-arrayexpress

grape leaves polyphenols inhibit breast cancer malignancy

Project description:We tested the inhibitory effects of hydroalcoholic extracts from grape leaves in breast cancer malignancy using MCF-7 and SKBR-3 cell lines.

2020-05-27 | PXD016748 | Pride

Enhancer analysis of HER2+ cell lines SKBR-3 (ER-) and BT474m1 (ER+) in response to lapatinib and JQ1 and analysis of FOXA1 chromatin binding in SKBR-3 cells in response to lapatinib and JQ1 and impact of FOXA1 knockdown on BRD4 and MED1

Project description:SKBR-3 and BT474m1 HER2+ breast cancer cell lines were treated with lapatinib, JQ1, or lapatinib and JQ1 or (for SKBR-3) treated with siRNA pools (non-targeting control or FOXA1) prior to drug treatment before being used for ChIPseq (BRD4, MED1, H3K27Ac, or FOXA1 (for SKBR-3))

2020-11-03 | GSE160667 | GEO

FOXA1-dependent responses of the HER2+ SKBR-3 cell line to lapatinib

Project description:SKBR-3 HER2+ breast cancer cells were treated with 25 nM Dharmacon siGENOME non-targeting (NT) siRNA control pool or siRNA pool targeting FOXA1 for 48h, then treated with either DMSO or 300nM lapatinib for 24h.

2020-10-26 | GSE160001 | GEO

Differential Targeting of the E-Cadherin and b-Catenin Complex by Gram-positive Probiotic Lactobacilli Improves Epithelial Barrier Function

Project description:The intestinal ecosystem is balanced by dynamic interactions between resident and incoming microbes, the gastrointestinal barrier, and the mucosal immune system. However, in the context of inflammatory bowel diseases (IBD) where the integrity of the gastrointestinal barrier is compromised, resident microbes contribute to the development and perpetuation of inflammation and disease. In this context, probiotic bacteria exert beneficial effects enhancing epithelial barrier integrity. However, the mechanisms underlying these beneficial effects are only poorly understood. Here, we comparatively investigated the effects of four probiotic lactobacilli, namely L. acidophilus, L. fermentum, L. gasseri, and L. rhamnosus in a T84 cell epithelial barrier model. Results of DNA-microarray experiments indicating that lactobacilli modulate the regulation of genes encoding in particular adherence junction proteins such as E-cadherin and b-catenin were confirmed by qRT-PCR. Furthermore, we show that epithelial barrier function is modulated by Gram-positive probiotic lactobacilli via their effect on adherence junction protein expression and complex formation. In addition, incubation with lactobacilli differentially influences the phosphorylation of adherence junction proteins and of PKC isoforms such as PKCd which thereby positively modulates epithelial barrier function. Further insight into the underlying molecular mechanisms triggered by these probiotics might also foster the development of novel strategies for the treatment of gastrointestinal diseases (e.g. IBD).

2012-02-10 | E-MEXP-3445 | biostudies-arrayexpress

The effect of knock-down of neuroblastoma associated lncRNA on transcriptome

Project description:As part of functional characterization of neuroblastoma assocated lncRNA, we performed its knock-down in neuroblastoma cell line SH-SY5Y, which resulted in modulation of expression levels of a set of genes involved in angiogenesis and inflammation, the hallmarks of metastatic cancer. SH-SY5Y cells were transfected with non-targeting siRNA control and two siRNAs targeting lncRNA BEHOT. Two days after transfection total RNA was isolated and hybridized to microarray, each sample was done in four replicas.

2013-04-01 | E-GEOD-40680 | biostudies-arrayexpress

Transcription profiling by array of human rheumatoid fibroblast-like synoviocytes to study the role of micro RNAs in IL-18 synthesis regulation

Project description:Study of the role of micro RNAs in IL-18 synthesis regulation in rheumatoid fibroblast-like synoviocytes (FLS)

2010-03-13 | E-MEXP-1970 | biostudies-arrayexpress

Transcription profiling of human MCF7 cell line with siRNA mediated knockdown of CNOT7 and/or CNOT8 subunits of Ccr4-Not deadenylase compared to control

Project description:siRNA-mediated knockdown of CNOT7 and/or CNOT8 compared to control to study role in the regulation of gene expression by these factors.

2009-07-15 | E-MEXP-2218 | biostudies-arrayexpress

RNAi profiling by array of human MCF7 cells with siRNA-mediated knockdown of CNOT6 and/or CNOT6L, or CNOT7 and CNOT8

Project description:siRNA-mediated knockdown of CNOT6 and/or CNOT6L, or CNOT7 and CNOT8 compared to control to study role in the regulation of gene expression by these factors.

2011-09-14 | E-MEXP-2926 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data