Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of blood from patients with HNSCC who either responded to radiotherapy with a high or low increase in plasma DROM and decrease in FRAP

ABSTRACT: The study was performed on a subgroup of a larger study including 78 patients with histologically verified HNSCC recruited from Division of Cancer Medicine and Radiotherapy, Norwegian Radium Hospital, Rikshospitalet University Hospital in the period from May 2003 to May 2006. The patients received either post-operative radiotherapy or radiotherapy alone for a period of 5-7 weeks.
Ten HNSCC patients, responding to radiotherapy with a high relative increase in plasma DROM and high decrease in FRAP (responder), were compared to ten patients with low increase in DROM and low decrease in FRAP (non responder). Microarray analysis of full blood were performed on pre and post radiotherapy samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Siv KjM-xlsrud BM-xhn

PROVIDER: E-MEXP-2460 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Stress associated gene expression in blood cells is related to outcome in radiotherapy treated head and neck cancer patients.

Bøhn Siv K SK Russnes Kjell M KM Sakhi Amrit K AK Thoresen Magne M Holden Marit M Moskaug Jan Ø JØ Myhrstad Mari C MC Olstad Ole K OK Smeland Sigbjørn S Blomhoff Rune R

BMC cancer 20120925

<h4>Background</h4>We previously observed that a radiotherapy-induced biochemical response in plasma was associated with favourable outcome in head and neck squamous carcinoma cancer (HNSCC) patients. The aim of the present study was to compare stress associated blood cell gene expression between two sub-groups of HNSCC patients with different biochemical responses to radiotherapy.<h4>Methods</h4>Out of 87 patients (histologically verified), 10 biochemical 'responders' having a high relative inc ...[more]

PMID: 23009663

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Project description:Background: MicroRNAs (miRNAs) are small, non-coding, RNA molecules which regulate numerous cellular processes. Specific miRNA may be abnormally down-regulated or up-regulated in colorectal cancer and have been found associated with prognosis or response to treatments. However, no study has ever addressed their predictive role in rectal cancer. Therefore, we used microarray technology and RT-PCR to profile miRNA expression patterns in patients (pts) with rectal cancer, with the aim to identify a specific M-bM-^@M-^\signatureM-bM-^@M-^] associated with pathological complete response after neoadjuvant chemo-radiotherapy. Methods: 38 pts with locally advanced rectal cancer (cT3-4/N+) were treated with capecitabine-oxaliplatin and pelvic conformal radiotherapy (45 cGy) followed by surgery (after 6-8 weeks). Pathologic response was scored according to the tumor regression grade (TRG) scale. MiRNA expression profile was analysed by microarray on fresh frozen biopsies obtained before treatment start and confirmed by RT-PCR. The correlation between miRNA expression profile and the TRG coded as TRG1 (pathologic Complete Response-pCR) versus TRG >1 (no pCR) was assessed by statistical analysis methods specifically designed for this study. Findings: 14 miRNAs were selected by arrays analysis as differentially expressed in TRG1 pts and 13 were confirmed by RT-PCR. In particular, 11 miRNAs (miR-1183, miR-483-5p, miR-622, miR-125a-3p, miR-1224-5p, miR-188-5p, miR-1471, miR-671-5p, miR-1909*, miR-630 and miR-765) were significantly up-regulated in TRG1 pts, while 2 miRNAs were under -expressed (miR-1274b and miR-720). miR-622 and miR-630 showed 100% sensitivity and specificity in selecting TRG1 cases and were significantly correlated with EGFR (M-OM-^G2=11M-bM-^@M-"793; p= 0M-bM-^@M-"001) and TS expression (M-OM-^G2=10M-bM-^@M-"589; p= 0M-bM-^@M-"001). Interpretation: A set of 13 miRNAs is strongly associated with pathologic complete response and may represent a specific marker of response to chemo-radiotherapy in locally advanced rectal cancer. Experiments were performed on purified RNA from preoperatory biopsies of 38 patients with histological diagnosis of rectal adenocarcinoma invading through the intestinal wall and/or with pelvic lymph node involvement as evaluated by endorectal ultrasonography (uT3-T4 and/or uN+). Patients were treated with neoadjuvant chemo-radiotherapy (capecitabine + oxaliplatin in combination with 45 Gy of pelvic conformal radiotherapy). Patients have been divided in the following two groups: group A those who had obtained a pathologic complete response M-bM-^@M-^S TRG 1, including the patient without detectable disease who refused surgery, group B any pathologic response other than complete.

Dataset Information

Transcription profiling of blood from patients with HNSCC who either responded to radiotherapy with a high or low increase in plasma DROM and decrease in FRAP

Publications

Stress associated gene expression in blood cells is related to outcome in radiotherapy treated head and neck cancer patients.

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