Project description:Investigation whether tracheal organ cultures (TOCs) are a suitable model for characterization of early immune response triggered by influenza virus infection in the respiratory tract. Comparison to in vivo infected animals.
Project description:Sylvia Reemers: chicken in vivo infection: timecourse, groupI= non immunised non challenged, groupIII= non immunised challenged, groupIV= immunised challenged, groupV= immunised+w/o challenged, groupVII= immunised+Al(OH)3 challenged, groupIX= immunised+CpG challenged Tissue type= Lung1. The aim of this study was to get a better understanding of the characteristics of a protective response and provide a signature of protection after vaccination with H9N2 adjuvanted with different immunomodulators.
Project description:Sylvia Reemers: chicken in vivo infection: timecourse, groupI= non immunised non challenged, groupIII= non immunised challenged, groupIV= immunised challenged, groupV= immunised+w/o challenged, groupVII= immunised+Al(OH)3 challenged, groupIX= immunised+CpG challenged Tissue type= Lung4. The aim of this study was to get a better understanding of the characteristics of a protective response and provide a signature of protection after vaccination with H9N2 adjuvanted with different immunomodulators.
Project description:Sylvia Reemers: chicken in vivo infection: timecourse, groupI= non immunised non challenged, groupIII= non immunised challenged, groupIV= immunised challenged, groupV= immunised+w/o challenged, groupVII= immunised+Al(OH)3 challenged, groupIX= immunised+CpG challenged Tissue type= Upper trachea. The aim of this study was to get a better understanding of the characteristics of a protective response and provide a signature of protection after vaccination with H9N2 adjuvanted with different immunomodulators.
Project description:To identify mRNA substrates for the human RNase MRP complex, we examined the effects of siRNA-mediated depletion of RNase MRP (and RNase P) on the transcriptome of HEp-2 cells. The expression of two RNase MRP protein components, hPop1 and Rpp40, was knocked-down and after 48 hours mRNA was isolated from these cells as well as from cells transfected with an siRNA targeting the GFP mRNA (siEGFP), which was used as a control. The expression levels of mRNAs were analyzed on a genome-wide scale using 21K microarrays.
Project description:analysis of differential gene expression in CD34+ derived neutrophil progenitors from umbilical cord blood, in response to Mek1 activation
Project description:Tissue specific microarray data is obtained by generating a whole-organism extract and subsequent co-immune precipitation of mRNA with the poly-A binding protein FLAG::PAB-1 specifically expressed in body wall muscle cells (Roy et al. 2002). Different lines were compared: SV911 (control), SV912 (CYE-1/CDK-2AF), and SV985 (CYD-1/CDK-4). As reference total RNA was used.