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Transcription profiling of mouse forelimb autopods and genital buds from wild type and homozygous Del9 E12.5 embryos

ABSTRACT: Despite the importance of Hox genes in patterning the mouse embryo, few target genes of the Hox transcription factors have been identified. To search for HoxD targets we contrasted gene expression profiles in the presence and absence of the HoxD genes in two tissues where these genes are important in embryonic patterning-the genital bud and the distal domain of the limb. The Del9 mutant, in which all nine HoxD genes are absent, shows perturbed digit and genital morphogenesis. Therefore we used Affymetrix GeneChip arrays to compare gene expression in forelimb autopods and genital buds from wild type and homozygous Del9 E12.5 embryos.

ORGANISM(S): Mus musculus

SUBMITTER: John Cobb

PROVIDER: E-MEXP-257 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparative analysis of genes downstream of the Hoxd cluster in developing digits and external genitalia.

Cobb John J Duboule Denis D

Development (Cambridge, England) 20050701 13

Mammalian Hox genes encode transcription factors that are crucial for proper morphogenesis along the various body axes. Despite their extensive structural and functional characterization, the nature of their target genes remains elusive. We have addressed this question by using DNA microarrays to screen for genes whose expression in developing distal forelimbs and genital eminences was significantly modified in the absence of the full Hoxd gene complement. This comparative approach not only iden ...[more]

PMID: 15944189

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